Free Wanderer Powder regulates AMPA receptor homeostasis in chronic restraint stress-induced rat model of depression with liver-depression and spleen-deficiency syndrome
Autor: | You-Ming Jiang, Shengyan Xi, Yingkun Qiu, Qun Liu, Tiegang Liu, Jing Shi, Yueyun Liu, Guang-Xin Yue, Jiaxu Chen, Yanan Wang, Zhigeng Li, Yuan Liang, Pei Ma, Lifeng Yue |
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Rok vydání: | 2019 |
Předmět: |
Aging
medicine.medical_specialty Hippocampus AMPA receptor Amygdala Western blot Internal medicine homeostasis medicine medicine.diagnostic_test business.industry musculoskeletal neural and ocular physiology Glutamate receptor chronic restraint stress Cell Biology Endocrinology medicine.anatomical_structure nervous system depression Immunohistochemistry free wanderer powder Glutamatergic synapse business Homeostasis Research Paper |
Zdroj: | Aging (Albany NY) |
ISSN: | 1945-4589 |
Popis: | Free Wanderer Powder (FWP) is a classic formula for depression with digestive dysfunctions, i.e., liver-depression and spleen-deficiency syndrome (LDSDS) in Chinese Medicine. But its protective mechanism has not been fully clarified. Here a chronic restraint stress (CRS) induced rat model showed depression with LDSDS in food intake, metabolism, and behaviour tests. Then 75 rats were randomly divided, and received CRS and different treatment with behaviour tests. Expressions of c-Fos and AMPA-type glutamate receptor subunits GluR1-3 in hippocampus CA1, CA3, DG and amygdala BLA were detected by immunohistochemistry, western blot and RT-PCR, respectively. In CRS rats, FWP alleviated depressive behaviour and c-Fos expression. FWP suppressed the increasement of GluR1 in CA1 and DG, p-GluR1 in CA1, and p-GluR2 and GluR3 in BLA. FWP also blocked the decrease of GluR1 and Glur2/3 in CA3, p-GluR1 in CA3, and p-GluR2 in CA1 and CA3. Furthermore, constituents of FWP and their potential targets were explored using UHPLC-MS and systematic bioinformatics analysis. There were 23 constituents identified in FWP, 9 of which regulated glutamatergic synapse. Together, these results suggest that FWP contains effective constituents and alleviates depression with LDSDS by regulating AMPA-type glutamate receptor homeostasis in amygdala and hippocampus. |
Databáze: | OpenAIRE |
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