One-shot analysis of translated mammalian lncrnas with aharibo
| Autor: | Fabio Lauria, Daniele Peroni, Francesco Gandolfi, Alessandro Romanel, Alessia Del Piano, Romina Belli, Simone Sidoli, Alberto Peretti, Gabriella Viero, Alessandro Quattrone, Jacopo Zasso, Massimiliano Clamer, Graziano Guella, Paola Bernabo, Claudia Firrito, Luca Minati |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Translation Mouse QH301-705.5 Science translation Computational biology Biology ENCODE Ribosome General Biochemistry Genetics and Molecular Biology Translatome Mice Animals RIBO-seq Biology (General) Proteogenomics One shot General Immunology and Microbiology General Neuroscience RNA Translation (biology) Mouse Embryonic Stem Cells General Medicine Cell Biology LncRNA Embryonic stem cell Tools and Resources ribosome Cytoplasm Protein Biosynthesis proteogenomics Medicine RNA Long Noncoding Mammalian genome Peptides Ribosomes Human |
| Zdroj: | eLife eLife, Vol 10 (2021) eLife 10 (2021): 1. doi:10.7554/eLife.59303 info:cnr-pdr/source/autori:Minati L.; Firrito C.; Del Piano A.; Peretti A.; Sidoli S.; Peroni D.; Belli R.; Gandolfi F.; Romanel A.; Bernabo P.; Zasso J.; Quattrone A.; Guella G.; Lauria F.; Viero G.; Clamer M./titolo:One-shot analysis of translated mammalian lncrnas with aharibo/doi:10.7554%2FeLife.59303/rivista:eLife/anno:2021/pagina_da:1/pagina_a:/intervallo_pagine:1/volume:10 |
| Popis: | SUMMARYA vast portion of the mammalian genome is transcribed as long non-coding RNAs (lncRNAs) acting in the cytoplasm with largely unknown functions. Surprisingly, lncRNAs have been shown to interact with ribosomes, encode uncharacterized proteins, or act as ribosome sponges. These functions still remain mostly undetected and understudied owing to the lack of efficient tools for genome-wide simultaneous identification of ribosome-associated lncRNAs and peptide-producing lncRNAs.Here we present AHARIBO, a method for the detection of lncRNAs either untranslated, but associated with ribosomes, or encoding small peptides. Using AHARIBO in mouse embryonic stem cells during neuronal differentiation, we isolated ribosome-protected RNA fragments, translated RNAs and correspondingde novosynthesized polypeptides. Besides identifying mRNAs under active translation and associated ribosomes, we found and distinguished lncRNAs acting as ribosome sponges or encoding micropeptides, laying the ground for a better functional understanding of hundreds lncRNAs. |
| Databáze: | OpenAIRE |
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