Intrinsic Depot-Specific Differences in the Secretome of Adipose Tissue, Preadipocytes, and Adipose Tissue–Derived Microvascular Endothelial Cells
| Autor: | David E. James, Samantha L. Hocking, Michael Guilhaus, Lindsay E. Wu, Donald J. Chisholm |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Angiogenesis Endocrinology Diabetes and Metabolism Adipose tissue macrophages Subcutaneous Fat Adipose tissue Adipokine Intra-Abdominal Fat Biology Mass Spectrometry Mice Insulin resistance Internal medicine Adipocytes Internal Medicine medicine Animals Amino Acids Microcirculation nutritional and metabolic diseases Proteins medicine.disease Peptide Fragments Culture Media Mice Inbred C57BL Platelet Endothelial Cell Adhesion Molecule-1 Endothelial stem cell Metabolism Endocrinology Secretory protein Adipose Tissue Endothelium Vascular Insulin Resistance Peptides tissues Explant culture |
| Zdroj: | Diabetes |
| ISSN: | 1939-327X 0012-1797 |
| DOI: | 10.2337/db10-0483 |
| Popis: | OBJECTIVE Visceral adipose tissue (VAT) is more closely linked to insulin resistance than subcutaneous adipose tissue (SAT). We conducted a quantitative analysis of the secretomes of VAT and SAT to identify differences in adipokine secretion that account for the adverse metabolic consequences of VAT. RESEARCH DESIGN AND METHODS We used lectin affinity chromatography followed by comparison of isotope-labeled amino acid incorporation rates to quantitate relative differences in the secretomes of VAT and SAT explants. Because adipose tissue is composed of multiple cell types, which may contribute to depot-specific differences in secretion, we isolated preadipocytes and microvascular endothelial cells (MVECs) and compared their secretomes to those from whole adipose tissue. RESULTS Although there were no discrete depot-specific differences in the secretomes from whole adipose tissue, preadipocytes, or MVECS, VAT exhibited an overall higher level of protein secretion than SAT. More proteins were secreted in twofold greater abundance from VAT explants compared with SAT explants (59% versus 21%), preadipocytes (68% versus 0%), and MVECs (62% versus 15%). The number of proteins in the whole adipose tissue secretome was greater than the sum of its cellular constituents. Finally, almost 50% of the adipose tissue secretome was composed of factors with a role in angiogenesis. CONCLUSIONS VAT has a higher secretory capacity than SAT, and this difference is an intrinsic feature of its cellular components. In view of the number of angiogenic factors in the adipose tissue secretome, we propose that VAT represents a more readily expandable tissue depot. |
| Databáze: | OpenAIRE |
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