Isolation and chacterisation of Klebsiella phages for phage therapy
| Autor: | Lucy Gannon, Slawomir Michniewski, Eleanor Jameson, Saija Kiljunen, Lucy Kelly, Rhys A. Dunstan, Andrew D. Millard, Eleanor M. Townsend, George Muscatt, Mikael Skurnik, Anna Kolsi, Trevor Lithgow, Hari Sapkota |
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| Přispěvatelé: | HUMI - Human Microbiome Research, Research Programs Unit, Department of Bacteriology and Immunology, HUSLAB, Helsinki University Hospital Area, Faculty of Medicine, University of Helsinki, Medicum, Mikael Skurnik / Principal Investigator, Helsinki One Health (HOH) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Klebsiella
phage therapy Phage therapy medicine.drug_class medicine.medical_treatment viruses Antibiotics antibiotics Microbiology Bacteriophage 03 medical and health sciences Antibiotic resistance bacteriophage phage Medicine characterization antimicrobial resistance Pathogen 030304 developmental biology 0303 health sciences biology 030306 microbiology business.industry biology.organism_classification Antimicrobial medicine.disease 3. Good health QR virulence nosocomial infection 3111 Biomedicine business Pneumonia (non-human) |
| Zdroj: | BASE-Bielefeld Academic Search Engine |
| ISSN: | 2641-6530 |
| Popis: | Introduction: Klebsiella is a clinically important pathogen causing a variety of antimicrobial resistant infections in both community and nosocomial settings, particularly pneumonia, urinary tract infection, and sepsis. Bacteriophage (phage) therapy is being considered a primary option for the treatment of drug-resistant infections of these types. Methods: We report the successful isolation and characterization of 30 novel, genetically diverse Klebsiella phages. Results: The isolated phages span six different phage families and nine genera, representing both lysogenic and lytic lifestyles. Individual Klebsiella phage isolates infected up to 11 of the 18 Klebsiella capsule types tested, and all 18 capsule-types were infected by at least one of the phages. Conclusions: Of the Klebsiella-infecting phages presented in this study, the lytic phages are most suitable for phage therapy, based on their broad host range, high virulence, short lysis period and given that they encode no known toxin or antimicrobial resistance genes. Phage isolates belonging to the Sugarlandvirus and Slopekvirus genera were deemed most suitable for phage therapy based on our characterization. Importantly, when applied alone, none of the characterized phages were able to suppress the growth of Klebsiella for more than 12 h, likely due to the inherent ease of Klebsiella to generate spontaneous phage-resistant mutants. This indicates that for successful phage therapy, a cocktail of multiple phages would be necessary to treat Klebsiella infections. |
| Databáze: | OpenAIRE |
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