Anticancer efficacy of 6-thioguanine loaded chitosan nanoparticles with or without curcumin
| Autor: | Muralidhar Metta, M Narayana Swamy, G Srinivasa Rao, V. Rama Devi, H.D. Narayanaswamy, KR Anjan Kumar, A.R. Gomes, CR Santhosh, G. P. Kalmath, N. Prakash, Prem Kumar Govindappa, Rashmi Rajashekaraiah, S. Wilfred Ruban, A. Sahadev, U Sunilchandra, H Dhanalakshmi, Suguna Rao, S. Naveen Kumar, K. Satyanarayan, Doddamane Rathnamma, P. Ravi Kumar |
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| Rok vydání: | 2020 |
| Předmět: |
Curcumin
Cell Survival Antineoplastic Agents Apoptosis 02 engineering and technology Biochemistry Chitosan 03 medical and health sciences chemistry.chemical_compound Structural Biology Cell Line Tumor Zeta potential Humans MTT assay Viability assay Particle Size Thioguanine Molecular Biology 030304 developmental biology Drug Carriers 0303 health sciences Cell Cycle Checkpoints General Medicine 021001 nanoscience & nanotechnology In vitro Drug Liberation chemistry Cell culture MCF-7 Cells Nanoparticles 0210 nano-technology Nuclear chemistry |
| Zdroj: | International Journal of Biological Macromolecules. 148:704-714 |
| ISSN: | 0141-8130 |
| Popis: | 6-Thioguanine encapsulated chitosan nanoparticles (6-TG-CNPs) has formulated by the ionic-gelation method. Morphologically, the 6-TG-CNPs were spherical and showed mean size, PDI, zeta potential, and entrapment efficiency of 261.63 ± 6.01 nm, 0.34 ± 0.10, +15.97 ± 0.46 mV and 44.27%, respectively. The IR spectra confirmed the 6-TG complex with chitosan. The in vitro drug release profile of 6-TG-CNPs revealed an increase in sustained-release (91.40 ± 1.08% at 48 h) at pH 4.8 compared to less sustained-release (73.96 ± 1.12% at 48 h) at pH 7.4. The MTT assay was conducted on MCF-7 and PA-1 cell lines at 48 h incubation to determine % cell viability. The IC50 values of 6-TG, 6-TG-CNPs, and curcumin for MCF-7 were 23.09, 17.82, and 15.73 μM, respectively. Likewise, IC50 values of 6-TG, 6-TG-CNPs, and curcumin for PA-1 were 5.81, 3.92, and 12.89 μM, respectively. A combination of 6-TG-CNPs (IC25) with curcumin (IC25) on PA-1 and MCF-7 showed % cell viability of 43.67 ± 0.02 and 49.77 ± 0.05, respectively. The in vitro cytotoxicity potential in terms of % cell viability, early apoptosis, G2/M phase arrest, and DNA demethylating activity of 6-TG-CNPs alone and combination with curcumin proved to be more effective than that of 6-TG on PA-1 cells. |
| Databáze: | OpenAIRE |
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