Investigation on mitochondrial tRNA(Leu/Lys), NDI and ATPase 6/8 in Iranian multiple sclerosis patients
| Autor: | Massoud Houshmand, Sadaf Kasraie, Mehdi Shafa Shariat Panahi, Solmaz Etemad Ahari, Mostafa Moin, Mohammad Ali Bahar |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Mitochondrial DNA
Multiple Sclerosis RNA Transfer Leu Protein subunit DNA Mutational Analysis Biology Iran DNA Mitochondrial Electron Transport Complex IV Cellular and Molecular Neuroscience medicine Demyelinating disease Humans Point Mutation Genetics ATP synthase Multiple sclerosis Cytochrome c oxidase subunit II Point mutation NADH Dehydrogenase Cell Biology General Medicine Mitochondrial Proton-Translocating ATPases medicine.disease Molecular biology Mitochondrial respiratory chain Case-Control Studies biology.protein |
| Zdroj: | Cellular and molecular neurobiology. 27(6) |
| ISSN: | 0272-4340 |
| Popis: | As with chromosomal DNA, the mitochondrial DNA (mtDNA) can contain mutations that are highly pathogenic . In fact, many diseases of the central nervous system are known to be caused by mutations in mtDNA. Dysfunction of the mitochondrial Respiratory Chain (RC) has been shown in patients with neurological disease including Alzheimer's disease (AD), Parkinson's disease (PD) and Multiple sclerosis (MS). MS is a demyelinating disease of central nervous system characterized by morphological hallmarks of inflammation, demyelination and axonal loss. Considering this importance, we decided to investigate several highly mutative parts of mtDNA for point mutations as MT-LTI (tRNA(Leucine1(UUA/G))), MT-NDI (NADH Dehydrogenase subunit 1), MT-COII (Cytochrome c oxidase subunit II), MT-TK (tRNA(Lysine)), MT-ATP8 (ATP synthase subunit F0 8) and MT-ATP6 (ATP synthase subunit F0 6) in 20 Iranian MS patients and 80 age-matched control subjects by PCR and automated DNA sequencing to evaluate any probable point mutations. Our results revealed that 15 (75%) out of 20 MS patients had point mutations. Some of point mutations were newly found in this study. This study suggested that point mutation occurred in mtDNA might be involved in pathogenesis of MS. |
| Databáze: | OpenAIRE |
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