The c-Ha-ras oncogene induces increased expression of β-galactoside α-2,6-sialyltransferase in rat fibroblast (FR3T3) cells
| Autor: | Vincent Laudet, Benoit van Hille, Nadia Le Marer, André Verbert, C. Lagrou, Dominique Stehelin, Haris Cazlaris, Jean Montreuil, Eric C. Svensson, Philippe Delannoy |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Sialyltransferase
Molecular Sequence Data Cell Fluorescent Antibody Technique Gene Expression Transfection Biochemistry Malignant transformation medicine Animals RNA Messenger Fibroblast beta-D-Galactoside alpha 2-6-Sialyltransferase Immunosorbent Techniques Cell Line Transformed Oncogene biology Chemistry Malignant Conversion Cell Membrane Galactosides Oncogenes Fibroblast growth factor receptor 4 Fibroblasts Fibroblast growth factor receptor 3 Molecular biology N-Acetylneuraminic Acid Sialyltransferases Rats Cell Transformation Neoplastic Genes ras medicine.anatomical_structure Carbohydrate Sequence Sialic Acids biology.protein Glycoconjugates |
| Zdroj: | Glycobiology. 2:49-56 |
| ISSN: | 1460-2423 0959-6658 |
| DOI: | 10.1093/glycob/2.1.49 |
| Popis: | Alteration in cell surface carbohydrates, and in particular cell surface sialylation, have been known to occur during oncogenic transformation. To examine the basis for such changes, we have transformed the rat fibroblast cell line FR3T3 with the oncogenes c-Ha-ras EJ, v-mycOK10, v-src, polyoma virus middle T or the transforming bovine papilloma virus 1 (BPV1), and measured the sialytransferase activities of cellular lysates. We found that, in contrast to all other oncogenes examined, c-Ha-ras induced a striking increase in beta-galactoside alpha-2,6-sialytransferase (Gal alpha-2,6-ST) activity in FR3T3 cells. This increase in Gal alpha-2,6-ST activity resulted in the increased expression of cell surface alpha-2,6-linked sialic acid on cell surface glycoconjugates, as determined by cell staining with fluorescein-labelled Sambucus nigra agglutinin. Immunoprecipitation and immunofluorescence experiments revealed that the increase in Gal alpha-2,6-ST activity was due to an elevation of expression of the enzyme. Moreover, Northern analysis suggested that the increased expression of this enzyme was the result of an increase in the steady-state mRNA level of the Gal alpha-2,6-ST gene. These results support the notion that alterations seen in cell surface glycoconjugates during oncogenic transformation can be the result of altered expression of glycosyltransferases. |
| Databáze: | OpenAIRE |
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