An infectious cDNA clone of a growth attenuated Korean isolate of MERS coronavirus KNIH002 in clade B
| Autor: | Jeong Sun Yang, Jae Su Moon, Geon-Woo Kim, Hee Cho, Woo Jung Park, Joo Yeon Lee, Sung Soon Kim, Jong Won Oh, Jang Hoon Choi, Minwoo Kim, Seung Hoon Lee, Jeong Min Kim, Wooseong Lee, Hae Gwang Jung |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
ORF5 deletion variants DNA Complementary Middle East respiratory syndrome coronavirus Epidemiology viruses 030106 microbiology Immunology Clone (cell biology) Biology medicine.disease_cause Microbiology Virus law.invention 03 medical and health sciences Viral Proteins MERS-CoV Molecular evolution law infectious cDNA clone Cell Line Tumor Cricetinae Virology Chlorocebus aethiops Drug Discovery medicine Animals Humans Clade Vero Cells Strain (biology) General Medicine Reverse genetics Clone Cells 030104 developmental biology Infectious Diseases Recombinant DNA Middle East Respiratory Syndrome Coronavirus Receptors Virus Parasitology growth attenuation clade B Korean isolate Research Article |
| Zdroj: | Emerging Microbes & Infections article-version (VoR) Version of Record |
| ISSN: | 2222-1751 |
| DOI: | 10.1080/22221751.2020.1861914 |
| Popis: | The MERS-CoV isolated during the 2015 nosocomial outbreak in Korea showed distinctive differences in mortality and transmission patterns compared to the prototype MERS-CoV EMC strain belonging to clade A. We established a BAC-based reverse genetics system for a Korean isolate of MERS-CoV KNIH002 in the clade B phylogenetically far from the EMC strain, and generated a recombinant MERS-CoV expressing red fluorescent protein. The virus rescued from the infectious clone and KNIH002 strain displayed growth attenuation compared to the EMC strain. Consecutive passages of the rescued virus rapidly generated various ORF5 variants, highlighting its genetic instability and calling for caution in the use of repeatedly passaged virus in pathogenesis studies and for evaluation of control measures against MERS-CoV. The infectious clone for the KNIH002 in contemporary epidemic clade B would be useful for better understanding of a functional link between molecular evolution and pathophysiology of MERS-CoV by comparative studies with EMC strain. |
| Databáze: | OpenAIRE |
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