Dextran sulfate sodium upregulates MAPK signaling for the uptake and subsequent intracellular survival of Brucella abortus in murine macrophages
Autor: | Wongi Min, Hannah Leah Tadeja Simborio, Huynh Tan Hop, Lauren Togonon Arayan, Hong Hee Chang, Dong Hee Kim, Hu Jang Lee, Suk Kim, Alisha Wehdnesday Bernardo Reyes |
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Rok vydání: | 2015 |
Předmět: |
0301 basic medicine
MAP Kinase Signaling System animal diseases Phagocytosis 030106 microbiology Brucella abortus Vacuole Brucella Microbiology Bacterial Adhesion Brucellosis 03 medical and health sciences Mice Downregulation and upregulation Macrophage Animals Humans Late endosome Innate immune system Microbial Viability biology Macrophages Dextran Sulfate bacterial infections and mycoses biology.organism_classification Up-Regulation 030104 developmental biology Infectious Diseases RAW 264.7 Cells Host-Pathogen Interactions Intracellular |
Zdroj: | Microbial pathogenesis. 91 |
ISSN: | 1096-1208 |
Popis: | Brucellosis is one of the major zoonoses worldwide that inflicts important health problems in animal and human. Here, we demonstrated that dextran sulfate sodium (DSS) significantly increased adhesion of Brucella (B.) abortus in murine macrophages compared to untreated cells. Even without infection, Brucella uptake into macrophages increased and F-actin reorganization was induced compared with untreated cells. Furthermore, DSS increased the phosphorylation of MAPKs (ERK1/2 and p38α) in Brucella-infected, DSS-treated cells compared with the control cells. Lastly, DSS markedly increased the intracellular survival of Brucella abortus in macrophages by up to 48 h. These results suggest that DSS enhanced the adhesion and phagocytosis of B. abortus into murine macrophages by stimulating the MAPK signaling proteins phospho-ERK1/2 and p38α and that DSS increased the intracellular survival of B. abortus by inhibiting colocalization of Brucella-containing vacuoles (BCVs) with the late endosome marker LAMP-1. This study emphasizes the enhancement of the phagocytic and intracellular modulatory effects of DSS, which may suppress the innate immune system and contribute to prolonged Brucella survival and chronic infection. |
Databáze: | OpenAIRE |
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