Expression and function of OXE receptor, an eicosanoid receptor, in steroidogenic cells
| Autor: | Paula Maloberti, Mariana Cooke, Hernán Di Cónsoli, Fabiana Cornejo Maciel |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Leukotrienes medicine.medical_specialty CIENCIAS MÉDICAS Y DE LA SALUD Docosahexaenoic Acids Transcription Genetic Eicosanoid receptor Gene Expression STEROIDOGENESIS Arachidonic Acids Biology Biochemistry Cell Line Mice Endocrinology 5-LIPOXYGENASE ARACHIDONIC ACID Internal medicine medicine Animals Humans RNA Messenger Molecular Biology Arachidonic Acid Liver receptor homolog-1 Leydig Cells Bioquímica y Biología Molecular Phosphoproteins OXOEICOSANOID RECEPTOR OXE receptor Medicina Básica Receptors Eicosanoid Adrenal Cortex Steroids |
| Zdroj: | Molecular and Cellular Endocrinology. 371:71-78 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/j.mce.2012.11.003 |
| Popis: | Hormonal regulation of steroidogenesis involves arachidonic acid (AA) metabolism through the 5-lipoxygenase pathway. One of the products, 5-hydroperoxy-eicosatetraenoic acid (5-HpETE), acts as a modulator of the activity of the steroidogenic acute regulatory (StAR) protein promoter. Besides, an oxoeicosanoid receptor of the leukotriene receptor family named OXE-R is a membrane protein with high affinity and response to 5-HpETE, among other AA derivatives. The aim of our work was to elucidate whether this receptor may be involved in steroidogenesis. RT-PCR and western blot analysis demonstrated the presence of the mRNA and protein of the receptor in human H295R adrenocortical cells. The treatment of H295R or MA-10 cells (murine Leydig cell line) with 8Br-cAMP together with docosahexaenoic acid (DHA, an antagonist of the receptor) partially reduced StAR induction and steroidogenesis. On the contrary, 5-oxo-ETE -the prototypical agonist, with higher affinity and potency on the receptor- increased cAMP-dependent steroid production, StAR mRNA and protein levels. These results lead us to conclude that AA might modulate StAR induction and steroidogenesis, at least in part, through 5-HpETE production and activation of a membrane receptor, such as the OXE-R Fil: Cooke, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina Fil: Di Consoli, Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina Fil: Maloberti, Paula Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina Fil: Cornejo Maciel, Maria Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina |
| Databáze: | OpenAIRE |
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