Human microglia cultures: a powerful model to study their origin and immunoreactive capacity
| Autor: | F.R. Buttarelli, D. Babiloni, G.M. Lauro, Valeria Sogos, F. Gremo, Maria Grazia Ennas, D. Cocchia, G. Starace |
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| Rok vydání: | 1995 |
| Předmět: |
Enzyme-Linked Immunosorbent Assay
Human leukocyte antigen Major histocompatibility complex Major Histocompatibility Complex Developmental Neuroscience Antigen Lectins MHC class I medicine Humans Cells Cultured MHC class II Glutathione Peroxidase Microglia biology Beta-2 microglobulin Lymphokine Brain Flow Cytometry Molecular biology Microscopy Electron medicine.anatomical_structure Fluorescent Antibody Technique Direct Immunology biology.protein Cell Division Developmental Biology |
| Zdroj: | International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience. 13(7) |
| ISSN: | 0736-5748 |
| Popis: | In this paper, we report that pure cultures of human microglia were obtained from long-term astrocytic cultures of human fetal brain. After five to six months and repeated cell passages, macrophage-like cells started to spontaneously form in vitro , so that in two to three weeks the whole culture was populated by them. These cells were grown up to over 50 passages in culture and analyzed for morphology, specific marker positivity, growth rate and major histocompatibility complex (MHC) antigen expression with or without gamma-interferon (IFN) stimulation. We found that, regardless of embryonic age of original cultures (10–15 weeks of gestation), cultures showed a remarkable homogeneity and purity and over 90 stained for typical microglial markers. Under basal conditions, two cell subpopulations similar to those described in vivo , we observed: the reactive ‘ameboid’ type and the resting ‘ramified’ one, the latter increasing with time in vitro and cell passages. Both cell subpopulations were capable of active phagocytosis and of high-rate proliferation. They spontaneously expressed low levels of MHC class II antigens, but were negative for MHC class I. Stimulation with gamma-interferon lymphokine upregulated the MHC class II expression as well as the MHC class I heavy chain form in ameboid, ‘reactive’ cells but not in the ramified ones. We also found that β 2 microglobulin, already expressed in basal conditions, was dissociated from HLA A-B-C molecules in lymphokine-stimulated cells at early passages. The physiological significance of these data, as well as the possible correlation with in vivo ontogenetic modifications, are also discussed. |
| Databáze: | OpenAIRE |
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