Comparative and functional triatomine genomics reveals reductions and expansions in insecticide resistance-related gene families
| Autor: | Flavio Francini, Sheila Ons, Andrés Lavore, Rolando Rivera-Pomar, Victorio Palacio, Mario H. Rodriguez, Ivana Sierra, Jesús Martínez-Barnetche, Lucila Traverso, Marcelo G. Lorenzo, Jose Manuel Latorre-Estivalis, Gastón Mougabure-Cueto |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Insecticides Epidemiology Biología Genome Insect Disease Vectors Toxicology Pathology and Laboratory Medicine purl.org/becyt/ford/1 [https] 0302 clinical medicine Cytochrome P-450 Enzyme System Invertebrate Genomics Medicine and Health Sciences Triatoma Animal Anatomy Phylogeny Genetics education.field_of_study Genome biology CHAGAS´S DISEASE Ecology Drosophila Melanogaster lcsh:Public aspects of medicine CYTOCHROMES P450 Agriculture Genomics Animal Models Bioquímica y Biología Molecular Insects Infectious Diseases Experimental Organism Systems Insect Proteins Drosophila Agrochemicals Detoxification Transcriptome Analysis CIENCIAS NATURALES Y EXACTAS Research Article Chagas’ disease Chagas disease lcsh:Arctic medicine. Tropical medicine Arthropoda GLUTATHIONE TRANSFERASES lcsh:RC955-962 030231 tropical medicine Population Research and Analysis Methods Ciencias Biológicas 03 medical and health sciences Model Organisms CARBOXYL-CHOLYNESTERASES parasitic diseases Triatoma infestans medicine Animals Animal Physiology Triatoma dimidiata purl.org/becyt/ford/1.6 [https] Trypanosoma cruzi Rhodnius prolixus education Organisms Public Health Environmental and Occupational Health Biology and Life Sciences Computational Biology lcsh:RA1-1270 Genome Analysis biology.organism_classification medicine.disease Invertebrates Insect Vectors 030104 developmental biology Animal Genomics Vector (epidemiology) INSECTICIDE RESISTANCE Antennae (Animal Physiology) Zoology |
| Zdroj: | PLoS Neglected Tropical Diseases, Vol 11, Iss 2, p e0005313 (2017) SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP PLoS Neglected Tropical Diseases CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1935-2735 |
| Popis: | Background: Triatomine insects are vectors of Trypanosoma cruzi, a protozoan parasite that is the causative agent of Chagas’ disease. This is a neglected disease affecting approximately 8 million people in Latin America. The existence of diverse pyrethroid resistant populations of at least two species demonstrates the potential of triatomines to develop high levels of insecticide resistance. Therefore, the incorporation of strategies for resistance management is a main concern for vector control programs. Three enzymatic superfamilies are thought to mediate xenobiotic detoxification and resistance: Glutathione Transferases (GSTs), Cytochromes P450 (CYPs) and Carboxyl/Cholinesterases (CCEs). Improving our knowledge of key triatomine detoxification enzymes will strengthen our understanding of insecticide resistance processes in vectors of Chagas’ disease. Methods and findings: The discovery and description of detoxification gene superfamilies in normalized transcriptomes of three triatomine species: Triatoma dimidiata, Triatoma infestans and Triatoma pallidipennis is presented. Furthermore, a comparative analysis of these superfamilies among the triatomine transcriptomes and the genome of Rhodnius prolixus, also a triatomine vector of Chagas’ disease, and other well-studied insect genomes was performed. The expression pattern of detoxification genes in R. prolixus transcriptomes from key organs was analyzed. The comparisons reveal gene expansions in Sigma class GSTs, CYP3 in CYP superfamily and clade E in CCE superfamily. Moreover, several CYP families identified in these triatomines have not yet been described in other insects. Conversely, several groups of insecticide resistance related enzymes within each enzyme superfamily are reduced or lacking in triatomines. Furthermore, our qRT-PCR results showed an increase in the expression of a CYP4 gene in a T. infestans population resistant to pyrethroids. These results could point to an involvement of metabolic detoxification mechanisms on the high levels of pyrethroid resistance detected in triatomines from the Gran Chaco ecoregion. Conclusions and significance: Our results help to elucidate the potential insecticide resistance mechanisms in vectors of Chagas’ disease and provide new relevant information for this field. This study shows that metabolic resistance might be a contributing cause of the high pyrethroid resistance observed in wild T. infestans populations from the Gran Chaco ecoregion, area in which although subjected to intense pyrethroid treatments, vector control has failed. This study opens new avenues for further functional studies on triatomine detoxification mechanisms. Centro Regional de Estudios Genómicos Centro de Endocrinología Experimental y Aplicada |
| Databáze: | OpenAIRE |
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