Identification of Beclin-1 from orange-spotted grouper (Epinephelus coioides) involved in viral infection
| Autor: | Yongxian Xu, Shina Wei, Zaohe Wu, Jichang Jian, Zihong Zou, Qiwei Qin, Jia Cai, Yishan Lu, Qi Zheng |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Fish Proteins
0301 basic medicine Programmed cell death Orange-spotted grouper Adaptive Immunity Aquatic Science Microbiology Fish Diseases 03 medical and health sciences Immune system Interferon medicine Animals Environmental Chemistry Grouper Amino Acid Sequence Pathogen Phylogeny Base Sequence biology Gene Expression Profiling Autophagy 04 agricultural and veterinary sciences General Medicine biology.organism_classification Immunity Innate 030104 developmental biology Gene Expression Regulation Viral replication 040102 fisheries 0401 agriculture forestry and fisheries Bass Beclin-1 Sequence Alignment medicine.drug |
| Zdroj: | Fish & Shellfish Immunology. 94:336-345 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2019.09.029 |
| Popis: | Beclin-1 is an essential autophagic regulator that plays diverse roles in physiology and disease. However, reports about the function of fish Beclin-1 during pathogen infection are still very limited. In this study, a Beclin-1 homolog (EcBeclin-1) from orange-spotted grouper (Epinephelus coioides) was identified and its roles in viral infection were investigated. EcBeclin-1 encoded 447amino acids protein with a BH3 domain, a CCD domain and an ECD domain, which shared high identities (97%–82%) with reported Beclin-1 proteins from mammal to fish. Quantitative real-time PCR (qRT-PCR) analysis revealed that EcBeclin-1 was predominantly expressed in brain and muscle of healthy grouper. Using fluorescence microscopy, we found that EcBeclin-1 was co-localized with endoplasmic reticulum (ER) in grouper spleen cells (EAGS). After red-spotted grouper nervous necrosis virus (RGNNV) infection in vitro, EcBeclin-1 transcript was significantly up-regulated, implying that EcBeclin-1 might be involved in viral infection. Furthermore, the in vitro studies of EcBeclin-1 overexpression promoted RGNNV induced autophagy, as well as the expression of coat protein (CP) and RNA-dependent RNA polymerase (RdRp). The overexpression of EcBeclin-1 suppressed the expressions of interferon pathway-related factors, inflammatory-related factors and activities of NF-κB and ISRE. Additionally, EcBeclin-1 could interact with EcBcl-xL in vitro. These data suggest that EcBeclin-1 affect viral replication through modulating IFN and inflammatory responses, as well as virus-induced cell death, which will help us to further explore the immune response of fish during viral infection. |
| Databáze: | OpenAIRE |
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