Cloning of ClC-2 chloride channel from murine duodenum and its presence in CFTR knockout mice
| Autor: | Byung Hee Han, Hyun Dju Kim, Lane L. Clarke, Nam Soo Joo, Leonard R. Forte |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
DNA
Complementary Duodenum Molecular Sequence Data Biophysics Cystic Fibrosis Transmembrane Conductance Regulator Biochemistry Mice Rapid amplification of cDNA ends Intestinal mucosa Chloride Channels Structural Biology Complementary DNA Genetics Animals Amino Acid Sequence Cloning Molecular Intestinal Mucosa Peptide sequence Mice Knockout Cloning chemistry.chemical_classification Base Sequence biology Reverse Transcriptase Polymerase Chain Reaction urogenital system Blotting Northern Molecular biology Cystic fibrosis transmembrane conductance regulator Amino acid CLC-2 Chloride Channels chemistry Chloride channel biology.protein RNA Sequence Alignment Plasmids |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1446:431-437 |
| ISSN: | 0167-4781 |
| Popis: | We report the cloning of a murine ClC-2 chloride channel cDNA from duodenal epithelium by reverse transcriptase-polymerase chain reaction (RT-PCR) using degenerate primers and by rapid amplification of cDNA ends (RACE)-PCR. Other than CFTR, this represents the first cloned chloride channel from intact intestine. The ClC-2 cDNA predicts encoding of a 908 amino acid polypeptide with a calculated M(r) of 99,373. The amino acid sequence of the murine ClC-2 chloride channel is over 94% identical to the ClC-2 chloride channel proteins of other species. Of interest is the finding that the ClC-2 mRNA is expressed about the same level in duodena from both CFTR knockout and wild-type mice. This is in keeping with the suggestion that ClC-2 might be a therapeutic target in cystic fibrosis. |
| Databáze: | OpenAIRE |
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