MULTILOCUS ENZYME ELECTROPHORESIS AND CYTOCHROME B GENE SEQUENCING–BASED IDENTIFICATION OF LEISHMANIA ISOLATES FROM DIFFERENT FOCI OF CUTANEOUS LEISHMANIASIS IN PAKISTAN
| Autor: | Paola Andrea Barroso, Yoshihisa Hashiguchi, Shigeo Nonaka, Abdul Manan Bhutto, Javed Hussain Baloch, Masataka Korenaga, Hiroshi Uezato, Jorge Diego Marco, Ken Katakura, Hirotomo Kato, Farooq Rahman Soomro |
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| Rok vydání: | 2006 |
| Předmět: |
Electrophoresis
Leishmaniasis Cutaneous Polymerase Chain Reaction DNA sequencing Microbiology law.invention Double-Blind Method Cutaneous leishmaniasis law Virology parasitic diseases medicine Animals Humans Pakistan Gene Phylogeny Polymerase chain reaction DNA Primers Leishmania biology Cytochrome b Kinetoplastida Sequence Analysis DNA Cytochromes b DNA Protozoan biology.organism_classification medicine.disease Enzymes Infectious Diseases Immunology Protozoa Parasitology |
| Zdroj: | Scopus-Elsevier ResearcherID Hirotomo Kato |
| ISSN: | 1476-1645 0002-9637 |
| DOI: | 10.4269/ajtmh.2006.75.261 |
| Popis: | Seventeen Leishmania stocks isolated from cutaneous lesions of Pakistani patients were studied by multilocus enzyme electrophoresis and by polymerase chain reaction amplification and sequencing of the cytochrome b (Cyt b) gene. Eleven stocks that expressed nine zymodemes were assigned to L. (Leishmania) major. All of them were isolated from patients in the lowlands of Larkana district and Sibi city in Sindh and Balochistan provinces, respectively. The remaining six, distributed in two zymodemes (five and one), isolated from the highland of Quetta city, Balochistan, were identified as L. (L.) tropica. The same result at species level was obtained by the Cyt b sequencing for all the stocks examined. No clear-cut association between the clinical features (wet or dry type lesions) and the Leishmania species involved was found. Leishmania (L.) major was highly polymorphic compared with L. (L.) tropica. This difference may be explained by the fact that humans may act as a sole reservoir of L. (L.) tropica in anthroponotic cycles; however, many wild mammals can be reservoirs of L. (L.) major in zoonotic cycles. |
| Databáze: | OpenAIRE |
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