Cryopreserved Dental Pulp Tissues of Exfoliated Deciduous Teeth Is a Feasible Stem Cell Resource for Regenerative Medicine

Autor: Songtao Shi, Lan Ma, Kazuaki Nonaka, Takayoshi Yamaza, Toshio Kukita, Kentaro Akiyama, Yusuke Makino, Haruyoshi Yamaza, Guangtai Song, Yoshihiro Hoshino
Rok vydání: 2012
Předmět:
Mice
Inbred MRL lpr
Pathology
Anatomy and Physiology
Mouse
medicine.medical_treatment
lcsh:Medicine
Autoimmunity
Tooth Exfoliation
Kidney Function Tests
Regenerative Medicine
Stem cell marker
Biochemistry
Cell Fate Determination
Regenerative medicine
Mice
0302 clinical medicine
Deciduous teeth
Biomechanics
Child
lcsh:Science
Musculoskeletal System
Minerals
0303 health sciences
Multidisciplinary
Stem Cells
Animal Models
Stem-cell therapy
3. Good health
Adult Stem Cells
medicine.anatomical_structure
Cellular Microenvironment
Child
Preschool
Female
Bone Diseases
Stem cell
Research Article
Biotechnology
Adult
Cell Physiology
medicine.medical_specialty
Tissue Mechanics
Bone and Mineral Metabolism
Immunology
Biology
Autoimmune Diseases
Immunomodulation
03 medical and health sciences
Model Organisms
medicine
Animals
Humans
Regeneration
Tooth
Deciduous
Bone regeneration
Dental Pulp
Cell Proliferation
030304 developmental biology
Cryopreservation
Tissue Engineering
Multipotent Stem Cells
lcsh:R
Mesenchymal stem cell
Mesenchymal Stem Cells
030206 dentistry
Metabolism
Th17 Cells
Pulp (tooth)
lcsh:Q
Biomarkers
Developmental Biology
Stem Cell Transplantation
Zdroj: PLoS ONE, Vol 7, Iss 12, p e51777 (2012)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0051777
Popis: Human exfoliated deciduous teeth have been considered to be a promising source for regenerative therapy because they contain unique postnatal stem cells from human exfoliated deciduous teeth (SHED) with self-renewal capacity, multipotency and immunomodulatory function. However preservation technique of deciduous teeth has not been developed. This study aimed to evaluate that cryopreserved dental pulp tissues of human exfoliated deciduous teeth is a retrievable and practical SHED source for cell-based therapy. SHED isolated from the cryopreserved deciduous pulp tissues for over 2 years (25-30 months) (SHED-Cryo) owned similar stem cell properties including clonogenicity, self-renew, stem cell marker expression, multipotency, in vivo tissue regenerative capacity and in vitro immunomodulatory function to SHED isolated from the fresh tissues (SHED-Fresh). To examine the therapeutic efficacy of SHED-Cryo on immune diseases, SHED-Cryo were intravenously transplanted into systemic lupus erythematosus (SLE) model MRL/lpr mice. Systemic SHED-Cryo-transplantation improved SLE-like disorders including short lifespan, elevated autoantibody levels and nephritis-like renal dysfunction. SHED-Cryo amended increased interleukin 17-secreting helper T cells in MRL/lpr mice systemically and locally. SHED-Cryo-transplantation was also able to recover osteoporosis bone reduction in long bones of MRL/lpr mice. Furthermore, SHED-Cryo-mediated tissue engineering induced bone regeneration in critical calvarial bone-defect sites of immunocompromised mice. The therapeutic efficacy of SHED-Cryo transplantation on immune and skeletal disorders was similar to that of SHED-Fresh. These data suggest that cryopreservation of dental pulp tissues of deciduous teeth provide a suitable and desirable approach for stem cell-based immune therapy and tissue engineering in regenerative medicine.
Databáze: OpenAIRE
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