Effects of ethanol and nicotine co-administration on follicular atresia and placental histo-morphology in the first-generation mice pups during intrauterine development and lactation periods

Autor: Massood Ezzatabadipour, Tahereh Haghpanah, Elahe Musanejad, Vida Mirzaie
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Bcl-2
B-cell lymphoma 2
BAX
BCL2 Associated X
Health
Toxicology and Mutagenesis
Placenta
010501 environmental sciences
Toxicology
01 natural sciences
Nicotine
DNA
Deoxyribonucleic acid
0302 clinical medicine
RA1190-1270
Lactation
HCL
Hydrogen Chloride
E2
Estradiol
s.c.
subcutaneously
AMH
Anti-Müllerian hormone
ANOVA
analysis of variance
Follicular atresia
Regular Article
H&E
Hematoxylin and Eosin
Ca2+
calcium
ELISA
enzyme-linked immunosorbent assay
PBS
Phosphate-buffered saline
medicine.anatomical_structure
OS
oxidative stress
Veh
vehicle
Ni
nicotine
NMRI
Naval Medical Research Institute
IUGR
intrauterine growth restriction
cat.no
catalogue number
EtOH
ethanol
medicine.drug
GD
gestation day
Offspring
PND
Postnatal day
ROS
reactive-oxygen-species
Ovary
nAChRs
nicotinic acetylcholine receptors
SEM
standard error of the mean
WHO
World Health Organization
Andrology
03 medical and health sciences
TUNEL
Terminal deoxynucleotidyl transferase dUTP nick end labeling
medicine
OFR
ovarian follicular reservoir
SPSS
statistical package for the social sciences
BMP
Bone morphogenetic protein
CTL
control
0105 earth and related environmental sciences
Pregnancy
Ethanol
business.industry
Histology
i.p.
intraperitoneally
medicine.disease
First-generation mice
Toxicology. Poisons
FSH
Follicle-stimulating hormone
business
030217 neurology & neurosurgery
DAPI
4′
6-diamidino-2-phenylindole
Zdroj: Toxicology Reports
Toxicology Reports, Vol 8, Iss, Pp 793-803 (2021)
ISSN: 2214-7500
Popis: Graphical abstract Schematic representation of experimental design in different groups of mothers and offspring. CTL group: control group; Veh group: vehicle group received normal saline; Ni group: nicotine group received 1 mg/kg/day nicotine, subcutaneously; EtOH group: ethanol group received 3 g/kg/day, 20 % v/v ethanol intraperitoneally; Ni + EtOH group: ethanol plus nicotine which received both.
Highlights • Ovarian weight reduced following pre and postnatal exposure to nicotine and ethanol. • Ovaries of exposed pups revealed a marked increase in apoptotic follicles. • Sex hormones; FSH and E2 and AMH levels were not significantly changed. • Following maternal exposure, placenta morpho-histology properties were changed.
This study is evaluating the effects of ethanol and nicotine exposure during pregnancy and lactation on placenta histology and follicular atresia in the first-generation (f1) mice pups. The experimental groups were 5 groups of NMRI pregnant mice, including: control, vehicle (received normal saline) ethanol (3 g/kg/day, 20 % v/v intraperitoneally), nicotine (1 mg/kg/day, subcutaneously), and ethanol plus nicotine which received both. Pregnant animals in each group were then divided into two groups, one group for examining the placenta that was treated for 18 days and the other group for the ovary of one-day-old (PND1) and fifty-six-day-old (PND56) female offspring who were treated for 42 days (during intrauterine development and lactation). After the autopsy procedure, histopathological and morphometrical observations were done. Data revealed that the exposed mice had a significant change in the placenta morphometry and histology as well as a marked increase in the number of ovarian TUNEL positive cells on postnatal days 1 and 56. Therefore, maternal exposure to alcohol and nicotine during developmental and lactation periods could lead to changes in the placenta properties as well as an increase in the apoptotic ovarian follicles in f1 mice pups.
Databáze: OpenAIRE
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