DYS STR analysis with epithelial cells in a rape case
| Autor: | Werner Pflug, Gerhard Bäßler, Xenia Steil, Gerda Weyermann, Andrea Betz, Gabriele Dietl |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty education Biology Y chromosome Pathology and Forensic Medicine law.invention law Y Chromosome medicine Humans Typing Saliva Polymerase chain reaction Genetics Medical jurisprudence Haplotype Epithelial Cells social sciences DNA Forensic Medicine DNA Fingerprinting humanities DNA profiling STR analysis Haplotypes Tandem Repeat Sequences Rape Microsatellite Female Law |
| Zdroj: | Forensic science international. 118(2-3) |
| ISSN: | 0379-0738 |
| Popis: | We report here the application of Y-chromosomal DNA analysis in a rape case, which occurred in Stuttgart, Germany. Microscopic examination of the victim's vaginal swabs and her underwear showed no sperm cells. DNA was extracted from vaginal and epithelial cells and analysed with the autosomal systems SE33, THO1 (singleplex) and with the multiplex Profiler Plus (Applied Biosystems, Foster City, USA). The results of these autosomal STR analysis gave no hint at a mixed sample and failed to identify a male profile. DYS STR analysis with the systems DYS391, DYS392, DYS393, DYS19 and DYS389 I/II showed the same characteristic features as the suspect. We used this incomplete haplotype to search in the Y-STR Haplotype Reference Database via Internet. In a Caucasian population sample of 3589 minimal haplotypes we found 71 matches. The suspect confessed the crime and was finally condemned to 4 years imprisonment. |
| Databáze: | OpenAIRE |
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