Tire debris organic extract affects Xenopus development
| Autor: | Renato Bacchetta, Paride Mantecca, Maurizio Gualtieri, Giovanni Vailati, Marina Camatini, Claudio Vismara, Manuela Andrioletti |
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| Přispěvatelé: | Mantecca, P, Gualtieri, M, Andrioletti, M, Bacchetta, R, Vismara, C, Vailati, G, Camatini, M |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Male
Tail Histology Necrosis Embryo Nonmammalian Polymers Xenopus Tire debris organic extract Microphthalmia Andrology Xenopus laevis Teratogenicity Hemiterpenes Pentanes medicine Butadienes Ecotoxicology Animals Eye Abnormalities lcsh:Environmental sciences General Environmental Science lcsh:GE1-350 biology Embryo FETAX Anatomy biology.organism_classification medicine.disease Teratology Teratogens Liver Toxicity Environmental Pollutants Female Particulate Matter medicine.symptom |
| Zdroj: | Environment International, Vol 33, Iss 5, Pp 642-648 (2007) |
| ISSN: | 0160-4120 |
| Popis: | Tire debris (TD) and its organic components were identified as a main source of PM10 atmospheric and water pollution. Because few data are available on the embryotoxic effects of TD organic components, the lethal and teratogenic potential of tire debris organic extract (TDOE) was evaluated using the frog embryo teratogenesis assay-Xenopus (FETAX), coupled with a histopathological screening of the survived larvae. From stage 8 to stage 47, Xenopus laevis embryos were exposed to TDOE at concentrations of 50, 80, 100, 120 and 140 mg/L. The results showed 50 mg/L TDOE to be the non-observable effect concentration (NOEC). TDOE mortality at 80 mg/L was significantly higher than the control, but did not increase further with higher concentrations. A good concentration-response was observed for percentages of malformed larva and from 80 mg/L on these percentages were significantly higher than the control. Therefore, probit analysis gave a 144.6 mg/L TC50. At 120 and 140 mg/L, many larvae were plurimalformed. The most frequent alterations observed were abnormal gut coiling, microphthalmia, monolateral anophthalmia, and narrowing eyes. The histological screening mainly revealed ocular malformations such as double retina, retina nervous cell layer coiling, and altered lens. Moreover severe vacuolisation and necrosis were scored in liver and axial musculature. These results strongly support the assumption that TDOE is a powerful teratogen for X. laevis. Keywords: Tire debris organic extract, FETAX, Histology, Teratogenicity |
| Databáze: | OpenAIRE |
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