Evaluation of 99mTc-UBI 29-41 scintigraphy for specific detection of experimental Staphylococcus aureus prosthetic joint infections
| Autor: | Alain Meulemans, Azzam Saleh-Mghir, Geneviève Martet, Dominique Le Guludec, Françoise Chau, O. Raguin, Jean-Marc Vrigneaud, Rachida Lebtahi, Laure Sarda-Mantel, Mick M. Welling, Florence Hervatin |
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| Rok vydání: | 2007 |
| Předmět: |
Staphylococcus aureus
Prosthetic joint Specific detection Staphylococcal infections Scintigraphy medicine.disease_cause Microbiology medicine Animals Gamma Cameras Radiology Nuclear Medicine and imaging Radionuclide imaging Radioactive Tracers Radionuclide Imaging medicine.diagnostic_test business.industry Extremities Organotechnetium Compounds General Medicine Staphylococcal Infections medicine.disease Antimicrobial Peptide Fragments Prosthesis Failure Rabbit model Female Joints Rabbits business Antimicrobial Cationic Peptides |
| Zdroj: | European Journal of Nuclear Medicine and Molecular Imaging. 34:1302-1309 |
| ISSN: | 1619-7089 1619-7070 |
| DOI: | 10.1007/s00259-007-0368-7 |
| Popis: | (99m)Tc-UBI 29-41 (UBI), an antimicrobial peptide, specifically targets bacteria. We tested the ability of UBI to discriminate between infected and uninfected prosthetic joints using a rabbit model previously validated.Left knee arthroplasty was performed on 20 New Zealand rabbits, then 10(7) cfu of S. aureus (n = 12) or sterile saline (n = 8) was injected into the joint. On days 9 and 20 after surgery, planar UBI scintigraphy was performed in six infected and four uninfected rabbits, 1 h and 4 h p.i. (150 MBq), on a gamma camera. Operated-to-normal knee activity ratio (ONKR) was calculated on each scintigram. Then, after sacrifice, tissue samples of both knees were counted in a gamma counter.One rabbit injected with sterile saline had cutaneous infection at sacrifice and was excluded from analysis. ONKR was higher in infected than in uninfected animals 4 h p.i. 20 days after surgery: 1.75 +/- 0.48 vs 1.13 +/- 0.11, p = 0.04. From 1 h to 4 h p.i., ONKR increased in 9/12 infected and 0/7 uninfected animals. According to UBI uptake intensity and kinetics, scintigraphy was truly positive in all infected cases on day 9 and in four of six infected cases on day 20. It was truly negative in two of three sterile inflamed prosthetic knees on day 9, and in all cases on day 20. Biodistribution studies revealed increased UBI uptake in periprosthetic tissues in all animals 9 days after surgery, and only in infected animals on day 20.In this experimental study, (99m)Tc-UBI 29-41 scintigraphy permitted the early detection of acute prosthetic joint infection, and exclusion of infection in chronic sterile prosthetic joint inflammation. |
| Databáze: | OpenAIRE |
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