Gas chromatography method for the separation of amino acids enantiomers in plasma and urine. Application in a case of short bowel syndrome
Autor: | Sybe K. Wadman, S.B. Van der Meer, L. J. M. Spaapen, D. Ketting, Marinus Duran |
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Rok vydání: | 1991 |
Předmět: |
Adult
Short Bowel Syndrome Aminoisobutyric Acids Chromatography Gas Adolescent Clinical Biochemistry Urine Biochemistry Excretion chemistry.chemical_compound Enterobacteriaceae Reference Values medicine Humans Lactic Acid Amino Acids Derivatization Child Alanine chemistry.chemical_classification Chromatography Chemistry Biochemistry (medical) Infant Stereoisomerism General Medicine Short bowel syndrome medicine.disease Amino acid Intestines Child Preschool Lactates Gas chromatography Trifluoroacetic anhydride |
Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 204(1-3) |
ISSN: | 0009-8981 |
Popis: | Urinary amino acids were isolated from the urine of healthy controls and a patient with a short bowel syndrome. Following derivatization with isopropylalcohol/HCl and trifluoroacetic anhydride the amino acid enantiomers were separated by gas chromatography on a Chirasil- l -Val column. All subjects excreted d -alanine (10–30% of total Ala). The percentage d -alanine was higher in the patient with the short bowel syndrome. The excretion of d -alanine did not correlate with the d -lactate excretion. An intestinal origin for the d -amino acids is the most probable explanation. |
Databáze: | OpenAIRE |
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