Gas chromatography method for the separation of amino acids enantiomers in plasma and urine. Application in a case of short bowel syndrome

Autor: Sybe K. Wadman, S.B. Van der Meer, L. J. M. Spaapen, D. Ketting, Marinus Duran
Rok vydání: 1991
Předmět:
Zdroj: Clinica chimica acta; international journal of clinical chemistry. 204(1-3)
ISSN: 0009-8981
Popis: Urinary amino acids were isolated from the urine of healthy controls and a patient with a short bowel syndrome. Following derivatization with isopropylalcohol/HCl and trifluoroacetic anhydride the amino acid enantiomers were separated by gas chromatography on a Chirasil- l -Val column. All subjects excreted d -alanine (10–30% of total Ala). The percentage d -alanine was higher in the patient with the short bowel syndrome. The excretion of d -alanine did not correlate with the d -lactate excretion. An intestinal origin for the d -amino acids is the most probable explanation.
Databáze: OpenAIRE
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