Cartilage acidic protein 1 promotes increased cell viability, cell proliferation and energy metabolism in primary human dermal fibroblasts
| Autor: | Sophia Letsiou, Henrique L. Gomes, Liliana Anjos, Deborah M. Power, Rute C. Félix, João C.R. Cardoso, Ana L. G. Mestre |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell Survival Immunofluorescence Biochemistry Extracellular matrix 03 medical and health sciences Cell Movement Gene expression Extracellular medicine Humans Cell migration Viability assay Cells Cultured Primary human fibroblasts Cell proliferation Cell Proliferation Skin 030102 biochemistry & molecular biology medicine.diagnostic_test Cell growth Chemistry Regeneration (biology) Calcium-Binding Proteins General Medicine In vitro scratch assay Fibroblasts ECIS biosensor Extracellular Matrix Cell biology hCRTAC1 030104 developmental biology Energy Metabolism |
| Zdroj: | Biochimie Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | Cartilage acidic protein 1 (CRTAC1) is an extracellular matrix protein of human chondrogenic tissue that is also present in other vertebrates, non-vertebrate eukaryotes and in some prokaryotes. The function of CRTAC1 remains unknown but the protein's structure indicates a role in cell-cell or cell-matrix interactions and calcium-binding. The aim of the present study was to evaluate the in vitro effects of hCRTAC1-A on normal human dermal fibroblasts (NHDF). A battery of in vitro assays (biochemical and PCR), immunofluorescence and a biosensor approach were used to characterize the protein's biological activities on NHDF cells in a scratch assay. Gene expression analysis revealed that hCRTAC1-A protein is associated with altered levels of expression for genes involved in the processes of cell proliferation (CXCL12 and NOS2), cell migration (AQP3 and TNC), and extracellular matrix-ECM regeneration and remodeling (FMOD, TIMP1, FN1) indicating a role for hCRTAC1-A in promoting these activities in a scratch assay. In parallel, the candidate processes identified by differential gene transcription were substantiated and extended using Electric cell-substrate impedance sensing (ECIS) technology, immunofluorescence and cell viability assays. Our findings indicate that hCRTAC1-A stimulated cell proliferation, migration and ECM production in primary human fibroblasts in vitro. (C) 2020 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved. H2020-MSCA-RISE-2015 ALGAE4A-B [691102] EMBRIC (European Union's Horizon 2020 research and innovation programme) [654008] Portuguese Foundation for Science and Technology (FCT)Portuguese Foundation for Science and Technology [UIDB/04326/2020] operational programme CRESC Algarve 2020 [EMBRC.PT ALG-01-0145-FEDER-022121] operational programme COMPETE 2020 [EMBRC.PT ALG-01-0145-FEDER-022121] FCTPortuguese Foundation for Science and TechnologyEuropean Commission [UIDB/04326/2020, DL57/2016/CP1361, CT0020, CT0011] info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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