Effect of 1,25-dihydroxy-vitamin D3 in experimental sepsis
| Autor: | Klaus Olgaard, Claus Hemmingsen, Søren Møller, Finn Laigaard |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Vitamin
Lipopolysaccharides Male calcitriol medicine.medical_specialty Calcitriol 25 Vitamin D Sepsis sepsis chemistry.chemical_compound Internal medicine Vitamin D and neurology Medicine Animals Rats Wistar coagulation Cecum Ligation thrombocytes Calcium metabolism Prothrombin time medicine.diagnostic_test business.industry General Medicine Vitamins Disseminated Intravascular Coagulation medicine.disease Angiotensin II Rats Endocrinology chemistry Immunology Liver function business medicine.drug Research Paper |
| Zdroj: | International Journal of Medical Sciences |
| ISSN: | 1449-1907 |
| Popis: | Background: In addition to the regulation of calcium homeostasis, vitamin D affects the cellular immune system, targets the TNF-α pathway and increases vasoconstrictor response to angiotensin II. We therefore examined the effect of 1,25-dihydroxy-vitamin D3 on coagulation and organ failure in experimental sepsis in the rat. Methods: Three series of placebo-controlled studies were conducted. All rats were pre-treated with daily SC injections of 1,25-dihydroxy-vitamin D3 100 ng/kg or placebo vehicle for 3 days. In study 1, sepsis was accomplished by abdominal surgery comprising a coecal ligation and puncture with a 1,2 mm needle, or sham surgery. In study 2, the rats had a single IP injection of lipopolysaccharide from E. Coli 0111:B4 (LPS) 8 mg/kg, or placebo. In study 3, an hour-long IV infusion of LPS 7 mg/kg, or placebo was given. Results: All three models of sepsis showed significant effects on coagulation and liver function with reduced thrombocyte count and prothrombin time together with elevated ALT and bilirubin (p |
| Databáze: | OpenAIRE |
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