Microphotometric scanning of chromatid gaps and breaks induced by AluI and BamHI in Chinese hamster ovary cells

Autor:	Wilner Martínez-López, Gustavo A. Folle, Máximo E. Drets, Rossana Bonomi
Jazyk:	angličtina
Rok vydání:	1996
Předmět:	biology Chinese hamster ovary cell Local organization Chromosome microphotometric scanning biology.organism_classification Molecular biology Chinese hamster Chromatin Restriction enzyme ovary cells chromatid gaps Genetics Chromatid AluI BamHI
Zdroj:	Brazilian Journal of Genetics, Volume: 19, Issue: 4, Pages: 577-582, Published: 1996 Brazilian Journal of Genetics v.19 n.4 1996 Brazilian Journal of Genetics Sociedade Brasileira de Genética (SBG) instacron:SBG
Popis:	Chromatid gaps and breaks induced by the restriction endonucleases AluI and BamHI in the long arm of chromosome 1 of Chinese hamster ovary cells were microphotometrically scanned and mapped to a quantitative G-band map. More than 50% of chromatid breaks appeared as chromatin losses of greater than 5% of the total arm length. The majority of chromatid gaps and breaks as well as chromatin losses induced by both restriction endonucleases were non-randomly located in a region from 0.35 to 0.65 relative length units of the long arm of chromosome 1. We suggest that the access of these endonucleases to chromosomal DNA depends on the local organization of the chromatin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb2b20a3707fea88b25c493a476b4742 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400007&lng=en&tlng=en Zobrazit plný text záznamu