Different types of FCgamma-receptors are involved in anti-Lewis Y antibody induced effector functions in vitro
| Autor: | M Dettke, H Loibner |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Cancer Research
medicine.medical_treatment Immunoglobulin E Lymphocyte Activation Monocytes Mice Immune system Lewis Blood Group Antigens Neoplasms FcγR medicine Tumor Cells Cultured Animals Humans Effector functions Receptor biology Chimera Tumor Necrosis Factor-alpha Monocyte Receptors IgG Antibody-Dependent Cell Cytotoxicity Antibodies Monoclonal Regular Article In vitro Cell biology medicine.anatomical_structure Cytokine Oncology Immunoglobulin G TNF-α Immunology biology.protein Antibody ADCC anti-LeY antibody |
| Zdroj: | British Journal of Cancer |
| ISSN: | 0007-0920 |
| Popis: | Stimulation of monocytes by interaction of monoclonal antibodies (mAbs) with Fc gamma receptors (FcgammaRs) results in the activation of various monocyte effector functions. In the present investigation we show that the anti-Lewis Y (LeY) anti-tumour mAb ABL 364 and its mouse/human IgG1 chimaera induce both antibody-dependent cellular cytotoxicity (ADCC) and the release of tumour necrosis factor alpha (TNF-alpha) during mixed culture of monocytes with LeY+ SKBR5 breast cancer cells in vitro. Although anti-LeY mAb-mediated TNF-alpha release paralleled ADCC activity, cytokine release required a higher concentration of sensitizing mAb than the induction of cytolysis. The determination of the FcgammaR classes involved in the induction of the distinct effector functions showed that anti-LeY mAb-induced cytolysis was triggered by interaction between anti-LeY mAbs and FcgammaRI. In contrast, mAb-induced TNF-alpha release mainly depended on the activation of monocyte FcgammaRII. Neutralization of TNF-alpha showed no influence on monocyte ADCC activity towards SKBR5 target cells. Our data indicate an independent regulation of anti-LeY mAb induced effector functions of ADCC and TNF-alpha release which seemed to be triggered by activation of different types of FcgammaR. |
| Databáze: | OpenAIRE |
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