Melanopsin+RGCs Are Fully Resistant to NMDA-Induced Excitotoxicity
Autor: | Beatriz Vidal-Villegas, María Paz Villegas-Pérez, Manuel Vidal-Sanz, Arturo Ortín-Martínez, Nicolás Cuenca Navarro, Jose Manuel Bernal-Garro, Juan A Miralles de Imperial-Ollero, Francisco M. Nadal-Nicolás, Johnny Di Pierdomenico |
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Přispěvatelé: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología, Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio 'Ramón Margalef', Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS) |
Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Retinal Ganglion Cells
retina genetic structures Excitotoxicity Cell Count Brn3a+RGCs medicine.disease_cause Melanopsin-RGCs Rats Sprague-Dawley lcsh:Chemistry chemistry.chemical_compound 0302 clinical medicine adult albino rat lcsh:QH301-705.5 Spectroscopy Transcription Factor Brn-3A Chemistry General Medicine Computer Science Applications medicine.anatomical_structure Intravitreal Injections melanopsin-RGCs NMDA receptor Female excitotoxicity SD-OCT Melanopsin medicine.medical_specialty N-Methylaspartate Adult albino rat Biología Celular Receptors N-Methyl-D-Aspartate Retinal ganglion Article Retina Catalysis Inorganic Chemistry 03 medical and health sciences In vivo Ophthalmology medicine Animals Physical and Theoretical Chemistry Intrinsically photosensitive-RGCs Molecular Biology Organic Chemistry Rod Opsins Glaucoma Retinal eye diseases ophthalmology glaucoma lcsh:Biology (General) lcsh:QD1-999 NMDA intrinsically photosensitive-RGCs 030221 ophthalmology & optometry sense organs 030217 neurology & neurosurgery Ex vivo |
Zdroj: | International Journal of Molecular Sciences Volume 20 Issue 12 International Journal of Molecular Sciences, Vol 20, Iss 12, p 3012 (2019) RUA. Repositorio Institucional de la Universidad de Alicante Universidad de Alicante (UA) |
Popis: | We studied short- and long-term effects of intravitreal injection of N-methyl-d-aspartate (NMDA) on melanopsin-containing (m+) and non-melanopsin-containing (Brn3a+) retinal ganglion cells (RGCs). In adult SD-rats, the left eye received a single intravitreal injection of 5µ L of 100nM NMDA. At 3 and 15 months, retinal thickness was measured in vivo using Spectral Domain-Optical Coherence Tomography (SD-OCT). Ex vivo analyses were done at 3, 7, or 14 days or 15 months after damage. Whole-mounted retinas were immunolabelled for brain-specific homeobox/POU domain protein 3A (Brn3a) and melanopsin (m), the total number of Brn3a+RGCs and m+RGCs were quantified, and their topography represented. In control retinas, the mean total numbers of Brn3a+RGCs and m+RGCs were 78,903 ± 3572 and 2358 ± 144 (mean ± SD n = 10), respectively. In the NMDA injected retinas, Brn3a+RGCs numbers diminished to 49%, 28%, 24%, and 19%, at 3, 7, 14 days, and 15 months, respectively. There was no further loss between 7 days and 15 months. The number of immunoidentified m+RGCs decreased significantly at 3 days, recovered between 3 and 7 days, and were back to normal thereafter. OCT measurements revealed a significant thinning of the left retinas at 3 and 15 months. Intravitreal injections of NMDA induced within a week a rapid loss of 72% of Brn3a+RGCs, a transient downregulation of melanopsin expression (but not m+RGC death), and a thinning of the inner retinal layers. |
Databáze: | OpenAIRE |
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