Melanopsin+RGCs Are Fully Resistant to NMDA-Induced Excitotoxicity

Autor: Beatriz Vidal-Villegas, María Paz Villegas-Pérez, Manuel Vidal-Sanz, Arturo Ortín-Martínez, Nicolás Cuenca Navarro, Jose Manuel Bernal-Garro, Juan A Miralles de Imperial-Ollero, Francisco M. Nadal-Nicolás, Johnny Di Pierdomenico
Přispěvatelé: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología, Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio 'Ramón Margalef', Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Retinal Ganglion Cells
retina
genetic structures
Excitotoxicity
Cell Count
Brn3a+RGCs
medicine.disease_cause
Melanopsin-RGCs
Rats
Sprague-Dawley

lcsh:Chemistry
chemistry.chemical_compound
0302 clinical medicine
adult albino rat
lcsh:QH301-705.5
Spectroscopy
Transcription Factor Brn-3A
Chemistry
General Medicine
Computer Science Applications
medicine.anatomical_structure
Intravitreal Injections
melanopsin-RGCs
NMDA receptor
Female
excitotoxicity
SD-OCT
Melanopsin
medicine.medical_specialty
N-Methylaspartate
Adult albino rat
Biología Celular
Receptors
N-Methyl-D-Aspartate

Retinal ganglion
Article
Retina
Catalysis
Inorganic Chemistry
03 medical and health sciences
In vivo
Ophthalmology
medicine
Animals
Physical and Theoretical Chemistry
Intrinsically photosensitive-RGCs
Molecular Biology
Organic Chemistry
Rod Opsins
Glaucoma
Retinal
eye diseases
ophthalmology
glaucoma
lcsh:Biology (General)
lcsh:QD1-999
NMDA
intrinsically photosensitive-RGCs
030221 ophthalmology & optometry
sense organs
030217 neurology & neurosurgery
Ex vivo
Zdroj: International Journal of Molecular Sciences
Volume 20
Issue 12
International Journal of Molecular Sciences, Vol 20, Iss 12, p 3012 (2019)
RUA. Repositorio Institucional de la Universidad de Alicante
Universidad de Alicante (UA)
Popis: We studied short- and long-term effects of intravitreal injection of N-methyl-d-aspartate (NMDA) on melanopsin-containing (m+) and non-melanopsin-containing (Brn3a+) retinal ganglion cells (RGCs). In adult SD-rats, the left eye received a single intravitreal injection of 5µ
L of 100nM NMDA. At 3 and 15 months, retinal thickness was measured in vivo using Spectral Domain-Optical Coherence Tomography (SD-OCT). Ex vivo analyses were done at 3, 7, or 14 days or 15 months after damage. Whole-mounted retinas were immunolabelled for brain-specific homeobox/POU domain protein 3A (Brn3a) and melanopsin (m), the total number of Brn3a+RGCs and m+RGCs were quantified, and their topography represented. In control retinas, the mean total numbers of Brn3a+RGCs and m+RGCs were 78,903 ±
3572 and 2358 ±
144 (mean ±
SD
n = 10), respectively. In the NMDA injected retinas, Brn3a+RGCs numbers diminished to 49%, 28%, 24%, and 19%, at 3, 7, 14 days, and 15 months, respectively. There was no further loss between 7 days and 15 months. The number of immunoidentified m+RGCs decreased significantly at 3 days, recovered between 3 and 7 days, and were back to normal thereafter. OCT measurements revealed a significant thinning of the left retinas at 3 and 15 months. Intravitreal injections of NMDA induced within a week a rapid loss of 72% of Brn3a+RGCs, a transient downregulation of melanopsin expression (but not m+RGC death), and a thinning of the inner retinal layers.
Databáze: OpenAIRE