Role of calcineurin in Ca2+-induced release of catecholamines and neuropeptides
| Autor: | P.N.E. de Graan, W.E.J.M. Ghijsen, M. de Wit, R. Kissmehl, A.G.M. Leenders, Jacques J. H. Hens, Victor M. Wiegant, Hendrikus Boddeke, W.H. Gispen, Ulrich Weller |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Male
Synaptobrevin CYCLOSPORINE-A Phosphatase Calcineurin Inhibitors B-50 GAP-43 Biology dynamin I Biochemistry BRAIN NERVE-TERMINALS Exocytosis Synaptotagmin 1 Sincalide phosphatase Geneeskunde Cellular and Molecular Neuroscience Norepinephrine Bacterial Proteins PERMEATED SYNAPTOSOMES Animals rat NEUROTRANSMITTER RELEASE MARCKS Enzyme Inhibitors Rats Wistar PROTEIN-KINASE-C Dynamin Calcineurin TRANSMITTER RELEASE DYNAMIN-I Synapsin Phosphoric Monoester Hydrolases Rats INDUCED NORADRENALINE RELEASE Immunoglobulin G Streptolysins Calcium exocytosis CALMODULIN-BINDING Synaptosomes |
| Zdroj: | Journal of neurochemistry, 71(5), 1978. Blackwell Science Journal of Neurochemistry, 71(5), 1978-1986. Blackwell Publishing Ltd |
| ISSN: | 0022-3042 |
| Popis: | Neurotransmission requires rapid docking, fusion, and recycling of neurotransmitter vesicles. Several of the proteins involved in this complex Ca2+-regulated mechanism have been identified as substrates for protein kinases and phosphatases, e.g., the synapsins, synaptotagmin, rabphilin3A, synaptobrevin, munc18, MARCKS, dynamin I, and B-50/GAP-43. So far most attention has focused on the role of kinases in the release processes, but recent evidence indicates that phosphatases may be as important. Therefore, we investigated the role of the Ca2+/calmodulin-dependent protein phosphatase calcineurin in exocytosis and subsequent vesicle recycling. Calcineurin-neutralizing antibodies, which blocked dynamin I dephosphorylation by endogenous synaptosomal calcineurin activity, but had no effect on the activity of protein phosphatases 1 or 2A, were introduced into rat permeabilized nerve terminals and inhibited Ca2+-induced release of [H-3]noradrenaline and neuropeptide cholecystokinin-8 in a specific and concentration-dependent manner. Our data show that the Ca2+/calmodulin-dependent phosphatase calcineurin plays an essential role in exocytosis and/or vesicle recycling of noradrenaline and cholecystokinin-8, transmitters stored in large dense-cored vesicles. |
| Databáze: | OpenAIRE |
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