Structure and properties of luciferase from Photobacterium phosphoreum
| Autor: | Stefano Ferri, Robert R. Soly, Rose Szittner, Edward A. Meighen |
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| Rok vydání: | 1991 |
| Předmět: |
Macromolecular Substances
Photobacterium phosphoreum Protein subunit Molecular Sequence Data Restriction Mapping Biophysics Biology Biochemistry Sequence Homology Nucleic Acid Luciferase Nucleotide Amino Acid Sequence Luciferases Molecular Biology Gene chemistry.chemical_classification Base Sequence Photobacterium Temperature Nucleic acid sequence Cell Biology biology.organism_classification Amino acid Kinetics chemistry Genes Bacterial Protein Biosynthesis |
| Zdroj: | Biochemical and Biophysical Research Communications. 176:541-548 |
| ISSN: | 0006-291X |
| Popis: | The nucleotide sequences of the luxA and luxB genes coding for the alpha and beta subunits, respectively, of luciferase from Photobacterium phosphoreum have been determined. The predicted amino acid sequences of the alpha and beta subunits were shown to be significantly different from other bacterial luciferases with 62 to 88% identity with the alpha subunits and 47 to 71% identity with the beta subunits of other species. Expression of the different luciferases appear to correlate with the number of modulator codons. Kinetic properties of P. phosphoreum luciferase were shown to reflect the bacterium's natural cold temperature habitat. |
| Databáze: | OpenAIRE |
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