RAPTA-Decorated Polyacrylamide Nanoparticles: Exploring their Synthesis, Physical Properties and Effect on Cell Viability
| Autor: | Benjamin S. Murray, Jordon Sandland, Huguette Savoie, Ross W. Boyle |
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| Rok vydání: | 2020 |
| Předmět: |
Cell Survival
Polyacrylamide Acrylic Resins Nanoparticle chemistry.chemical_element Antineoplastic Agents Adenocarcinoma 010402 general chemistry 01 natural sciences Biochemistry chemistry.chemical_compound Organometallic Compounds Tumor Cells Cultured Humans Colorectal adenocarcinoma Viability assay Cytotoxicity Molecular Biology 010405 organic chemistry Organic Chemistry 0104 chemical sciences Ruthenium chemistry Biophysics Molecular Medicine Nanomedicine Nanoparticles Ruthenium Compounds Colorectal Neoplasms Conjugate |
| Zdroj: | Chembiochem : a European journal of chemical biology. 22(5) |
| ISSN: | 1439-7633 |
| Popis: | In this study, we report for the first time the successful immobilization of a known cytoactive [Ru(η6-arene)(C2O4)PTA] (RAPTA) complex to a biologically inert polyacrylamide nanoparticle support. The nanoparticles have been characterised by zetasizer analysis, UV-Vis, ATR-FT-IR, TGA, and ICP-MS to qualitatively and quantitatively confirm the presence of the metallodrug on the surface of the carrier. The native RAPTA complex required a concentration of 50 µM to produce a cell viability of 47.1 ± 2.1% when incubated with human Caucasian colorectal adenocarcinoma cells for 72 hr. Under similar conditions a cell viability of 45.1 ± 1.9% was obtained with 0.5 µM of RAPTA complex in its immobilized form. Therefore, conjugation of the RAPTA metallodrug to our nanoparticle carriers resulted in a significant 100-fold decrease in effective concentration of ruthenium required for a near identical biological effect on cell viability. |
| Databáze: | OpenAIRE |
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