LncRNA LINC01116 sponges miR-93-5p to promote cell invasion and migration in small cell lung cancer
Autor: | Guangyu Yang, Lei Xian, Feihai Liang, Wenzhou Liu |
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Rok vydání: | 2021 |
Předmět: |
Adult
Male STAT3 Transcription Factor Pulmonary and Respiratory Medicine Lung Neoplasms Survival Carcinogenesis Cell STAT3 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Movement Cell Line Tumor Glioma Gene expression medicine Humans miR-93-5p Lung cancer Aged Cell Proliferation 030304 developmental biology lcsh:RC705-779 Cell invasion 0303 health sciences Small cell lung cancer biology business.industry Cancer LINC01116 lcsh:Diseases of the respiratory system Middle Aged medicine.disease Small Cell Lung Carcinoma Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs medicine.anatomical_structure 030220 oncology & carcinogenesis Cancer research biology.protein Female RNA Long Noncoding business Research Article |
Zdroj: | BMC Pulmonary Medicine, Vol 21, Iss 1, Pp 1-6 (2021) BMC Pulmonary Medicine |
ISSN: | 1471-2466 |
Popis: | Background LINC01116 is a recently identified oncogenic lncRNA in glioma. Differential expression analysis using the public gene expression analysis tool GEPIA revealed the upregulation of LINC01116 in lung cancer. We studied the functions of LINC01116 in small cell lung cancer (SCLC). Methods The expression of LINC01116 in several types of cancer tissue and the paired non-tumor tissues was evaluated by GEPIA. The effects of the overexpression of LINC01116 and miR-93-5p on the expression of STAT3 were evaluated. The effects of the overexpression of LINC01116, miR-93-5p and STAT3 on SHP-77 cell behaviors were evaluated by Transwell assays. Results LINC01116 was highly expressed in SCLC and predicted poor survival. In SCLC tissues, the expression of LINC01116 was positively correlated with STAT3. Bioinformatics analysis revealed that miR-93-5p may target LINC01116. Overexpression of LINC01116 increased STAT3 but did not affect the expression of miR-93-5p. Transwell assay showed that LINC01116 and STAT3 increased cell invasion and migration rates. MiR-93-5p played an suppressed cell behaviors and suppressed the role of LINC01116. Conclusion Therefore, LINC01116 might upregulate STA3 by sponging miR-93-5p, thereby promoting cell invasion and migration in SCLC. |
Databáze: | OpenAIRE |
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