Chitosanase fromStreptomyces coelicolorA3(2): biochemical properties and role in protection against antibacterial effect of chitosan
| Autor: | Mariana Gabriela Ghinet, Ryszard Brzezinski, Rolf MorosoliR. Morosoli, Sébastien Roy, Marie-Ève Lacombe-Harvey, Dominic Poulin-Laprade |
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| Přispěvatelé: | Centre d'Étude et de Valorisation de la Diversité Microbienne, Département de Biologie, Université de Sherbrooke (UdeS)-Faculté des sciences, Institut Armand Frappier (INRS-IAF), Institut National de la Recherche Scientifique [Québec] (INRS)-Réseau International des Instituts Pasteur (RIIP) |
| Rok vydání: | 2010 |
| Předmět: |
Glycoside Hydrolases
MESH: Sequence Homology Amino Acid Molecular Sequence Data Chitin Streptomyces coelicolor MESH: Amino Acid Sequence macromolecular substances Biology Biochemistry Streptomyces MESH: Chitin Microbiology Chitosan 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases MESH: Anti-Bacterial Agents MESH: Glycoside Hydrolases Glycoside hydrolase Amino Acid Sequence Chitosanase MESH: Phylogeny MESH: Bacterial Proteins Molecular Biology Phylogeny 030304 developmental biology 0303 health sciences MESH: Molecular Sequence Data Sequence Homology Amino Acid 030306 microbiology technology industry and agriculture Cell Biology MESH: Streptomyces coelicolor Antimicrobial biology.organism_classification Anti-Bacterial Agents carbohydrates (lipids) MESH: Chitosan chemistry Bacteria |
| Zdroj: | Biochemistry and Cell Biology Biochemistry and Cell Biology, NRC Research Press, 2010, 88 (6), pp.907-16. ⟨10.1139/O10-109⟩ |
| ISSN: | 1208-6002 0829-8211 |
| DOI: | 10.1139/o10-109 |
| Popis: | International audience; Chitosan, an N-deacetylated derivative of chitin, has attracted much attention as an antimicrobial agent against fungi, bacteria, and viruses. Chitosanases, the glycoside hydrolases responsible for chitosan depolymerisation, are intensively studied as tools for biotechnological transformation of chitosan. The chitosanase CsnA (SCO0677) from Streptomyces coelicolor A3(2) was purified and characterized. CsnA belongs to the GH46 family of glycoside hydrolases. However, it is secreted efficiently by the Tat translocation pathway despite its similarity to the well-studied chitosanase from Streptomyces sp. N174 (CsnN174), which is preferentially secreted through the Sec pathway. Melting point determination, however, revealed substantial differences between these chitosanases, both in the absence and in the presence of chitosan. We further assessed the role of CsnA as a potential protective enzyme against the antimicrobial effect of chitosan. A Streptomyces lividans TK24 strain in which the csnA gene was inactivated by gene disruption was more sensitive to chitosan than the wild-type strain or a chitosanase-overproducing strain. This is the first genetic evidence for the involvement of chitosanases in the protection of bacteria against the antimicrobial effect of chitosan. |
| Databáze: | OpenAIRE |
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