Whitefly HES1 binds to the intergenic region of Tomato yellow leaf curl China virus and promotes viral gene transcription
| Autor: | He Wenze, Yu-Meng Wang, Shu-Sheng Liu, Xin-Tong Ye, Ya-Zhou He, Xiao-Wei Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Transcription
Genetic Genome Viral Whitefly Bemisia tabaci Genome Article Hemiptera 03 medical and health sciences Intergenic region Solanum lycopersicum Transcription (biology) Two-Hybrid System Techniques Virology Tobacco Animals Gene silencing Enhancer Plant Diseases 030304 developmental biology Genetics 0303 health sciences Host Microbial Interactions biology Tomato yellow leaf curl China virus 030302 biochemistry & molecular biology Begomovirus food and beverages biology.organism_classification Insect Vectors Viral replication Gene Knockdown Techniques HES1 Insect Proteins Transcription Factor HES-1 DNA Intergenic Viral gene transcription Protein Binding |
| Zdroj: | Virology |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2020.01.009 |
| Popis: | Intergenic region of begomovirus genome is vital to virus replication and viral gene transcription in plants. Previous studies have reported that Tomato yellow leaf curl China virus (TYLCCNV), a begomovirus, is able to accumulate and transcribe in its whitefly vector. However, the viral and host components that participate in begomovirus transcription in whiteflies are hitherto unknown. Using a yeast one-hybrid system, we identified >50 whitefly proteins that interacted with TYLCCNV intergenic region. Dual luciferase analysis revealed that one of the identified proteins, the hairy and enhancer of split homolog-1 (HES1), specifically bound to CACGTG motif in TYLCCNV intergenic region. Silencing HES1 decreased viral transcription, accumulation and transmission. These results demonstrate that the interactions between whitefly proteins and the intergenic region of TYLCCNV may contribute to viral transcription in the whitefly vector. Our findings offer valuable clues for the research and development of novel strategies to interfere with begomovirus transmission. |
| Databáze: | OpenAIRE |
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