The Necessity of OCT-4 and CDX2 for Early Development and Gene Expression Involved in Differentiation of Inner Cell Mass and Trophectoderm Lineages in Bovine Embryos
| Autor: | Takashi Fujii, Ken Sawai, Kazuki Takahashi, Tsutomu Hashizume, Soichi Kageyama, Natsuko Emura, Hiroki Hirayama, Nobuyuki Sakurai |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cellular differentiation Embryonic Development Oct-4 Biology 03 medical and health sciences 0302 clinical medicine Gene expression medicine Animals Inner cell mass CDX2 Transcription Factor Cell Lineage Blastocyst Cells Cultured Regulation of gene expression Blastocoel Gene Expression Regulation Developmental Cell Differentiation Cell Biology Embryo Mammalian Molecular biology digestive system diseases Trophoblasts Cell biology 030104 developmental biology medicine.anatomical_structure Blastocyst Inner Cell Mass embryonic structures Cattle Female Octamer Transcription Factor-3 030217 neurology & neurosurgery Developmental Biology Biotechnology |
| Zdroj: | Cellular Reprogramming. 18:309-318 |
| ISSN: | 2152-4998 2152-4971 |
| DOI: | 10.1089/cell.2015.0081 |
| Popis: | The functions of POU class 5 transcription factor 1 (Oct-4) and caudal-type homeobox 2 (Cdx2) in the differentiation of the murine inner cell mass (ICM) and trophectoderm (TE) have been described in detail. However, little is known about the roles of OCT-4 and CDX2 in preimplantation bovine embryos. To elucidate their functions during early development in bovine embryos, we performed OCT-4 and CDX2 downregulation using RNA interference. We injected OCT-4- or CDX2-specific short interfering RNAs (siRNAs) into bovine zygotes. The rate of blastocyst development of OCT-4-downregulated embryos was lower compared with uninjected or control siRNA-injected embryos. Gene expression analysis revealed decreased CDX2 and fibroblast growth factor 4 expression in OCT-4-downregulated embryos. CDX2-downregulated embryos developed to the blastocyst stage; however, in most cases, blastocoel formation was delayed. Gene expression analysis revealed decreased GATA3 expression and elevated NANOG expression in CDX2-downregulated embryos. In conclusion, OCT-4 and CDX2 are essential for early development and gene expression involved in differentiation of ICM and TE lineages in bovine embryos. |
| Databáze: | OpenAIRE |
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