The effects of cross‐linking a collagen‐elastin dermal template on scaffold bio‐stability and degradation
Autor: | Francia Ximena Escobar, Roxanne Parungao, Joanneke Maitz, Zhe Li, Yiwei Wang, Ali Fathi, Paul van Zuijlen, Peter K.M. Maitz |
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Přispěvatelé: | Plastic, Reconstructive and Hand Surgery, AMS - Tissue Function & Regeneration, Amsterdam Movement Sciences - Restoration and Development, Amsterdam Movement Sciences, Paediatric Surgery |
Rok vydání: | 2020 |
Předmět: |
Male
Scaffold Contraction (grammar) human dermal fibroblasts mouse model 0206 medical engineering Biomedical Engineering Neovascularization Physiologic Medicine (miscellaneous) 02 engineering and technology Biomaterials 03 medical and health sciences Cell Movement Tensile Strength skin regeneration Ultimate tensile strength medicine Animals Humans Fibroblast Cell Proliferation degradation 030304 developmental biology Mice Inbred BALB C 0303 health sciences Tissue Scaffolds integumentary system biology Chemistry Regeneration (biology) collagen-elastin scaffold Dermis Fibroblasts 020601 biomedical engineering In vitro Elastin Cross-Linking Reagents medicine.anatomical_structure Models Animal Biophysics biology.protein Cattle Collagen Wound healing Porosity cross-linking |
Zdroj: | Journal of Tissue Engineering and Regenerative Medicine, 14(9), 1189-1200. John Wiley and Sons Ltd Journal of tissue engineering and regenerative medicine, 14(9), 1189-1200. John Wiley and Sons Ltd Maitz, J, Wang, Y, Fathi, A, Ximena Escobar, F, Parungao, R, van Zuijlen, P, Maitz, P & Li, Z 2020, ' The effects of cross-linking a collagen-elastin dermal template on scaffold bio-stability and degradation ', Journal of Tissue Engineering and Regenerative Medicine, vol. 14, no. 9, pp. 1189-1200 . https://doi.org/10.1002/term.3082 |
ISSN: | 1932-7005 1932-6254 |
Popis: | MatriDerm is a collagen-elastin dermal template that promotes regeneration in full-thickness wound repair. Due to its noncross-linked status, MatriDerm biodegrades quickly in a wound. Facilitating vascularization and dermal repair, it is desirable for MatriDerm to remain present until the wound healing process is complete, optimizing tissue regeneration and reducing wound contraction. The aim of this study was to investigate the effect of cross-linking MatriDerm on its mechanical and biological properties and to enhance its regenerative functionality. MatriDerm was chemically cross-linked and characterized in comparison with noncross-linked MatriDerm. Scaffold properties including surface morphology, protein release and mechanical strength were assessed. Cell-scaffold interaction, cell proliferation and migration were examined using human dermal fibroblasts. Scaffold biodegradation and its impact on wound healing and contraction were studied in a mouse model. Results showed that cross-linked MatriDerm displayed a small reduction in pore size, significantly less protein loss and a threefold increase in tensile strength. A significant increase in fibroblast proliferation and migration was observed in cross-linked MatriDerm with reduced scaffold contraction in vitro. In the mouse model, noncross-linked MatriDerm was almost completely biodegraded after 14 days whereas cross-linked MatriDerm remained intact. No significant difference in wound contraction was found between scaffolds. In conclusion, cross-linked MatriDerm showed a significant increase in stability and strength, enhancing its durability and cell-scaffold interaction. in vivo analysis showed cross-linked MatriDerm had a reduced biodegradation rate with a similar host response. The extended structural integrity of cross-linked MatriDerm could potentially facilitate improved skin tissue regeneration, promoting the formation of a more pliable scar. |
Databáze: | OpenAIRE |
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