An inactivated hand-foot-and-mouth disease vaccine using the enterovirus71 (C4a) strain isolated from a Korean patient induces a strongimmunogenic response in mice
Autor: | Young Ki Choi, Sang-Won Lee, Jin-Won Kim, Ji-Yeon Hyeon, June-Woo Lee, Jung-Ah Lee, Heeji Lim, Hyejin Kim, Jung Sik Yoo, Hyun Ju In, Sang-Gu Yeo |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Physiology medicine.medical_treatment Monophosphoryl Lipid A lcsh:Medicine Graduates Antibodies Viral Biochemistry Immunologic Adjuvants Mice 0302 clinical medicine Immune Physiology Medicine and Health Sciences Enterovirus 71 Public and Occupational Health 030212 general & internal medicine Enzyme-Linked Immunoassays lcsh:Science Immune Response Vaccines Immunity Cellular Mice Inbred BALB C Immune System Proteins Multidisciplinary biology Immunogenicity Viral Vaccine Vaccination and Immunization Vaccination Infectious Diseases Models Animal Educational Status Female Adjuvant Research Article Infectious Disease Control Immunology Dose-Response Relationship Immunologic Research and Analysis Methods Alumni Microbiology Antibodies 03 medical and health sciences Adjuvants Immunologic Immunity Virology Republic of Korea medicine Animals Humans Antigens Immunoassays Immunization Schedule business.industry lcsh:R Biology and Life Sciences Proteins Viral Vaccines biology.organism_classification Antibodies Neutralizing Enterovirus A Human 030104 developmental biology Vaccines Inactivated Immunoglobulin G People and Places Inactivated vaccine Immunologic Techniques Population Groupings lcsh:Q Preventive Medicine Hand Foot and Mouth Disease business |
Zdroj: | PLOS ONE(12): 5 PLoS ONE, Vol 12, Iss 5, p e0178259 (2017) PLoS ONE |
Popis: | Enterovirus 71 (EV71) is a major causative agent of hand-foot-and-mouth disease (HFMD) frequently occurring in children. HFMD induced by EV71 can cause serious health problems and has been reported worldwide, particularly in the Asia-Pacific region. In this study, we assessed the immunogenicity of a formalin-inactivated HFMD vaccine using an EV71 strain (FI-EV71 C4a) isolated from a Korean patient. The vaccine candidate was evaluated in mice to determine the vaccination doses and vaccine schedules. BALB/c mice were intra-muscularly administered 5, 10, or 20 mu g FI-EV71 vaccine, followed by a booster 2 weeks later. EV71-specific antibodies and neutralizing antibodies were induced and maintained until the end of the experimental period in all vaccinated groups. To determine the effectiveness of adjuvant for the EV71 vaccine, three adjuvants, i.e., aluminium hydroxide gel, monophosphoryl lipid A, and polyinosinic-polycytidylic acid, were administered separately with the FI-EV71 vaccine to mice via the intramuscular route. Mice administered the FI-EV71 vaccine formulated with all three adjuvants induced a significantly increased antibody response compared with that of the single adjuvant groups. The vaccinated group with triple adjuvants exhibited more rapid induction of EV71-specific and neutralizing antibodies than the other groups. These results suggested that the role of adjuvant in inactivated vaccine was important for eliciting effective immune responses against EV71. In conclusion, our results showed that FI-EV71 was a potential candidate vaccine for prevention of EV71 infection. |
Databáze: | OpenAIRE |
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