An inactivated hand-foot-and-mouth disease vaccine using the enterovirus71 (C4a) strain isolated from a Korean patient induces a strongimmunogenic response in mice

Autor: Young Ki Choi, Sang-Won Lee, Jin-Won Kim, Ji-Yeon Hyeon, June-Woo Lee, Jung-Ah Lee, Heeji Lim, Hyejin Kim, Jung Sik Yoo, Hyun Ju In, Sang-Gu Yeo
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Physiology
medicine.medical_treatment
Monophosphoryl Lipid A
lcsh:Medicine
Graduates
Antibodies
Viral
Biochemistry
Immunologic Adjuvants
Mice
0302 clinical medicine
Immune Physiology
Medicine and Health Sciences
Enterovirus 71
Public and Occupational Health
030212 general & internal medicine
Enzyme-Linked Immunoassays
lcsh:Science
Immune Response
Vaccines
Immunity
Cellular
Mice
Inbred BALB C
Immune System Proteins
Multidisciplinary
biology
Immunogenicity
Viral Vaccine
Vaccination and Immunization
Vaccination
Infectious Diseases
Models
Animal
Educational Status
Female
Adjuvant
Research Article
Infectious Disease Control
Immunology
Dose-Response Relationship
Immunologic
Research and Analysis Methods
Alumni
Microbiology
Antibodies
03 medical and health sciences
Adjuvants
Immunologic
Immunity
Virology
Republic of Korea
medicine
Animals
Humans
Antigens
Immunoassays
Immunization Schedule
business.industry
lcsh:R
Biology and Life Sciences
Proteins
Viral Vaccines
biology.organism_classification
Antibodies
Neutralizing
Enterovirus A
Human
030104 developmental biology
Vaccines
Inactivated
Immunoglobulin G
People and Places
Inactivated vaccine
Immunologic Techniques
Population Groupings
lcsh:Q
Preventive Medicine
Hand
Foot and Mouth Disease
business
Zdroj: PLOS ONE(12): 5
PLoS ONE, Vol 12, Iss 5, p e0178259 (2017)
PLoS ONE
Popis: Enterovirus 71 (EV71) is a major causative agent of hand-foot-and-mouth disease (HFMD) frequently occurring in children. HFMD induced by EV71 can cause serious health problems and has been reported worldwide, particularly in the Asia-Pacific region. In this study, we assessed the immunogenicity of a formalin-inactivated HFMD vaccine using an EV71 strain (FI-EV71 C4a) isolated from a Korean patient. The vaccine candidate was evaluated in mice to determine the vaccination doses and vaccine schedules. BALB/c mice were intra-muscularly administered 5, 10, or 20 mu g FI-EV71 vaccine, followed by a booster 2 weeks later. EV71-specific antibodies and neutralizing antibodies were induced and maintained until the end of the experimental period in all vaccinated groups. To determine the effectiveness of adjuvant for the EV71 vaccine, three adjuvants, i.e., aluminium hydroxide gel, monophosphoryl lipid A, and polyinosinic-polycytidylic acid, were administered separately with the FI-EV71 vaccine to mice via the intramuscular route. Mice administered the FI-EV71 vaccine formulated with all three adjuvants induced a significantly increased antibody response compared with that of the single adjuvant groups. The vaccinated group with triple adjuvants exhibited more rapid induction of EV71-specific and neutralizing antibodies than the other groups. These results suggested that the role of adjuvant in inactivated vaccine was important for eliciting effective immune responses against EV71. In conclusion, our results showed that FI-EV71 was a potential candidate vaccine for prevention of EV71 infection.
Databáze: OpenAIRE
Externí odkaz: