The effects of the covalent attachment of 3-(4-hydroxy-3,5-di-tert-butylphenyl) propyl amine to glutaraldehyde pretreated bovine pericardium on structural degeneration, oxidative modification, and calcification of rat subdermal implants
| Autor: | Harry Ischiropoulos, Robert J. Levy, Ivan S. Alferiev, Jeanne M. Connolly, Abigail J. Christian |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Antioxidant Materials science medicine.medical_treatment Biomedical Engineering Oxidative phosphorylation Article Biomaterials Hydroxyproline chemistry.chemical_compound medicine Animals chemistry.chemical_classification Metals and Alloys Prostheses and Implants medicine.disease Molecular biology In vitro Rats Amino acid Surgery chemistry Glutaral Ceramics and Composites Collagenase Cattle Glutaraldehyde Oxidation-Reduction Pericardium medicine.drug Calcification |
| Zdroj: | Journal of Biomedical Materials Research Part A. 103:2441-2448 |
| ISSN: | 1549-3296 |
| Popis: | Bioprosthetic heart valves (BHV) fabricated from glutaraldehyde pretreated heterograft materials, porcine aortic valves or bovine pericardium (BP), are widely used in cardiac surgery. BHV progressively fail in clinical use due to structural degeneration. Previously we reported that dityrosine, an oxidized amino acid, was present in failed clinical BP-BHV explants; unimplanted BP had no detectable dityrosine. In the same studies BP were demonstrated in vitro to be susceptible to oxidative damage, that could be mitigated with BP covalently modified with the antioxidant, 3-(4-hydroxy-3,5-di-tert-butylphenyl)propyl amine (DBP). The present studies compared in rat subdermal implants glutaraldehyde pretreated BP to BP modified with either DBP or the chemical reactions used to link DBP. All BP explants regardless of DBP demonstrated reduced hydroxyproline and increased digestibility by collagenase. However, the DBP-BP explants showed significant inhibition of reduced explant shrink temperatures (an index of crosslinking) as compared with control BP. Significant mitigation of calcification was observed in both the BP-DBP and chemically modified explants as compared with BP. Dityrosine was not detectable in the 90 day explants. It is concluded that rat subdermal BP implants undergo both calcific and noncalcific structural degeneration, but without the formation of dityrosine, unlike clinical BP explants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text