Follicle-like structures formed by intestinal cell lines derived from the HT29-D4 adenocarcinoma cell line: Morphological and functional characterization
| Autor: | Y. Barra, Monique Roccabianca, Christian Alquier, Jean Mauchamp, Odile Rimet, Aline Mirrione |
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| Přispěvatelé: | ESA CNRS 6032, Faculté de Pharmacie, 27, bd Jean‐Moulin, 13385 Marseille cedex 05, France |
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Anions
[SDV]Life Sciences [q-bio] Cell Lumen (anatomy) Fluorescent Antibody Technique Biology Rhodamine 123 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Cyclic AMP Humans Microscopy Phase-Contrast ATP Binding Cassette Transporter Subfamily B Member 1 MUC1 ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences Mucin Cell Membrane Colforsin Cell Polarity Water Biological Transport Epithelial Cells Cell Biology General Medicine Transfection Epithelium 3. Good health Cell biology Microscopy Electron medicine.anatomical_structure Biochemistry chemistry Cell culture 030220 oncology & carcinogenesis HT29 Cells |
| Zdroj: | Biology of the Cell Biology of the Cell, Wiley, 1999, 91 (2), pp.143-155. ⟨10.1016/s0248-4900(99)80038-0⟩ |
| ISSN: | 0248-4900 1768-322X |
| Popis: | When cultured in high glucose containing medium, the human colon carcinoma cell line HT29-D4 and a clone derived by transfection with the MDR1 cDNA (MDR31) form multilayers of unorganized cells which are not polarized and are linked by desmosomes. Within these multilayers appear spontaneously large multicellular follicle-like-structures (FLS) where polarized cells linked by tight junctional complexes surround a lumen. Electron microscopy showed that some FLS display well developed brush borders with densely packed microvilli. Others have irregularly oriented microvilli of various lengths or are even completely devoid of apical differentiation. The lumen contains a variable amount of amorphous osmiophilic material. The apical surface of FLS forming cells express dipeptidylpeptidase IV, carcinoembryonic antigen, the mucin MUC1 and for the transfected cells the gp-170 protein. The organic anion fluorescein is transported from the cell to the lumen of FLS. Rhodamine 123, a substrate of the gp-170 ABC transporter is also concentrated in the lumen formed by MDR31 cells. Verapamil and cyclosporine A inhibited this last transport. Cyclic AMP stimulates the formation of these structures since treatment of post-confluent multilayers dramatically increased the number of FLS in HT29-D4 and MDR31 cell cultures within 24 h. The spontaneous formation of these morphologically and functionally polarized structures appeared at random and might respond to the coincidence of fluctuating parameters of the regulatory pathways (cAMP, Ca2+). |
| Databáze: | OpenAIRE |
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