Genome-wide profiling of 24 hr diel rhythmicity in the water flea, Daphnia pulex: network analysis reveals rhythmic gene expression and enhances functional gene annotation
| Autor: | Tijana Milenkovic, Taryn Green, Melissa T. Stephens, Michael E. Pfrender, Boyoung Yoo, Giles E. Duffield, Aaron D. Sheppard, Erliang Zeng, Camille Alam, Gary F. George, Samuel S. C. Rund |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Periodicity Period (gene) Circadian clock Daphnia pulex Daphnia Arthropod Proteins 03 medical and health sciences Circadian Clocks Genetics Animals Gene Regulatory Networks Network centrality Diel vertical migration Diel Vertical Migration (DVM) Oligonucleotide Array Sequence Analysis biology Ecology Gene Expression Profiling Biological networks fungi Circadian Diel Molecular Sequence Annotation biology.organism_classification 030104 developmental biology Pulex Gene Expression Regulation Evolutionary biology Network clustering Gene expression DNA microarray Protein function prediction Algorithms Biological network Research Article Functional enrichment analysis Biotechnology |
| Zdroj: | BMC Genomics |
| ISSN: | 1471-2164 |
| Popis: | Background Marine and freshwater zooplankton exhibit daily rhythmic patterns of behavior and physiology which may be regulated directly by the light:dark (LD) cycle and/or a molecular circadian clock. One of the best-studied zooplankton taxa, the freshwater crustacean Daphnia, has a 24 h diel vertical migration (DVM) behavior whereby the organism travels up and down through the water column daily. DVM plays a critical role in resource tracking and the behavioral avoidance of predators and damaging ultraviolet radiation. However, there is little information at the transcriptional level linking the expression patterns of genes to the rhythmic physiology/behavior of Daphnia. Results Here we analyzed genome-wide temporal transcriptional patterns from Daphnia pulex collected over a 44 h time period under a 12:12 LD cycle (diel) conditions using a cosine-fitting algorithm. We used a comprehensive network modeling and analysis approach to identify novel co-regulated rhythmic genes that have similar network topological properties and functional annotations as rhythmic genes identified by the cosine-fitting analyses. Furthermore, we used the network approach to predict with high accuracy novel gene-function associations, thus enhancing current functional annotations available for genes in this ecologically relevant model species. Our results reveal that genes in many functional groupings exhibit 24 h rhythms in their expression patterns under diel conditions. We highlight the rhythmic expression of immunity, oxidative detoxification, and sensory process genes. We discuss differences in the chronobiology of D. pulex from other well-characterized terrestrial arthropods. Conclusions This research adds to a growing body of literature suggesting the genetic mechanisms governing rhythmicity in crustaceans may be divergent from other arthropod lineages including insects. Lastly, these results highlight the power of using a network analysis approach to identify differential gene expression and provide novel functional annotation. Electronic supplementary material The online version of this article (doi:10.1186/s12864-016-2998-2) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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