β-Adrenergic inhibition of contractility in L6 skeletal muscle cells
| Autor: | Tore Bengtsson, Anette I. Öberg, Nodi Dehvari |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Anatomy and Physiology
Potassium Channels Muscle Fibers Skeletal lcsh:Medicine Phosphatidylinositol 3-Kinases Cricetinae Molecular Cell Biology Myosin Cyclic AMP Signaling in Cellular Processes Phosphorylation lcsh:Science Musculoskeletal System Cells Cultured Protein Kinase C Calcium signaling rho-Associated Kinases Multidisciplinary Muscle Biochemistry Adrenergic beta-Agonists Potassium channel Erythromycin Cell biology medicine.anatomical_structure Muscle medicine.symptom Cell Movement Signaling Research Article Signal Transduction Muscle Contraction Muscle contraction medicine.medical_specialty CHO Cells Biology Contractility Cricetulus Internal medicine Cell Adhesion Cyclic GMP-Dependent Protein Kinases Extracellular medicine Animals Muscle Skeletal Cell adhesion lcsh:R Skeletal muscle Cyclic AMP-Dependent Protein Kinases Acetylcysteine Rats Endocrinology Calcium lcsh:Q Receptors Adrenergic beta-2 |
| Zdroj: | PLoS ONE, Vol 6, Iss 7, p e22304 (2011) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The β-adrenoceptors (β-ARs) control many cellular processes. Here, we show that β-ARs inhibit calcium depletion-induced cell contractility and subsequent cell detachment of L6 skeletal muscle cells. The mechanism underlying the cell detachment inhibition was studied by using a quantitative cell detachment assay. We demonstrate that cell detachment induced by depletion of extracellular calcium is due to myosin- and ROCK-dependent contractility. The β-AR inhibition of L6 skeletal muscle cell detachment was shown to be mediated by the β(2)-AR and increased cAMP but was surprisingly not dependent on the classical downstream effectors PKA or Epac, nor was it dependent on PKG, PI3K or PKC. However, inhibition of potassium channels blocks the β(2)-AR mediated effects. Furthermore, activation of potassium channels fully mimicked the results of β(2)-AR activation. In conclusion, we present a novel finding that β(2)-AR signaling inhibits contractility and thus cell detachment in L6 skeletal muscle cells by a cAMP and potassium channel dependent mechanism. |
| Databáze: | OpenAIRE |
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