Phaeobacter gallaeciensis genomes from globally opposite locations reveal high similarity of adaptation to surface life
| Autor: | Martine Berger, Heiko Liesegang, Torsten Thomas, Antje Wollherr, Sonja Voget, Thorsten Brinkhoff, Meinhard Simon, Tim Engelhardt, Staffan Kjelleberg, Sebastian Thole, Rolf Daniel, Daniela Kalhoefer |
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| Přispěvatelé: | School of Biological Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
DNA
Bacterial Genomics Microbiology Genome Synteny Bacterial Adhesion Tropolone 03 medical and health sciences 14. Life underwater Phaeobacter Rhodobacteraceae Ecology Evolution Behavior and Systematics 030304 developmental biology Genetics 0303 health sciences biology 030306 microbiology Australia Molecular Sequence Annotation Roseobacter biology.organism_classification Adaptation Physiological Science::Biological sciences [DRNTU] Spain Horizontal gene transfer Transposon mutagenesis Original Article Sequence Alignment Genome Bacterial |
| Popis: | Phaeobacter gallaeciensis, a member of the abundant marine Roseobacter clade, is known to be an effective colonizer of biotic and abiotic marine surfaces. Production of the antibiotic tropodithietic acid (TDA) makes P. gallaeciensis a strong antagonist of many bacteria, including fish and mollusc pathogens. In addition to TDA, several other secondary metabolites are produced, allowing the mutualistic bacterium to also act as an opportunistic pathogen. Here we provide the manually annotated genome sequences of the P. gallaeciensis strains DSM 17395 and 2.10, isolated at the Atlantic coast of north western Spain and near Sydney, Australia, respectively. Despite their isolation sites from the two different hemispheres, the genome comparison demonstrated a surprisingly high level of synteny (only 3% nucleotide dissimilarity and 88% and 93% shared genes). Minor differences in the genomes result from horizontal gene transfer and phage infection. Comparison of the P. gallaeciensis genomes with those of other roseobacters revealed unique genomic traits, including the production of iron-scavenging siderophores. Experiments supported the predicted capacity of both strains to grow on various algal osmolytes. Transposon mutagenesis was used to expand the current knowledge on the TDA biosynthesis pathway in strain DSM 17395. This first comparative genomic analysis of finished genomes of two closely related strains belonging to one species of the Roseobacter clade revealed features that provide competitive advantages and facilitate surface attachment and interaction with eukaryotic hosts. |
| Databáze: | OpenAIRE |
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