Rab39a and Rab39b display different intracellular distribution and function in sphingolipids and phospholipids transport

Autor: Julián Gambarte Tudela, Mariano Alonso Bivou, Ignacio Cebrian, María Teresa Damiani, Agustín Luján, Julio Buonfigli, Anahí Capmany
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
SPHINGOLIPIDS
Endocytic cycle
Golgi Apparatus
Chlamydia trachomatis
GTPase
Endoplasmic Reticulum
lcsh:Chemistry
0302 clinical medicine
lcsh:QH301-705.5
Spectroscopy
Phospholipids
RAB39
lipid transport
Chemistry
General Medicine
purl.org/becyt/ford/3.1 [https]
Computer Science Applications
Cell biology
Rab39
SMALL GTPASES
INTRACELLULAR TRAFFICKING
lipids (amino acids
peptides
and proteins)
purl.org/becyt/ford/3 [https]
LIPID TRANSPORT
Sphingomyelin
Intracellular
intracellular trafficking
Time-Lapse Imaging
small GTPases
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
RAB ISOFORMS
RAB PROTEINS
Humans
Physical and Theoretical Chemistry
CHLAMYDIA TRACHOMATIS
Molecular Biology
Lipid Transport
sphingolipids
Endoplasmic reticulum
Organic Chemistry
Biological Transport
Sphingolipid
Rab isoforms
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
rab GTP-Binding Proteins
Rab proteins
Rab
030217 neurology & neurosurgery
HeLa Cells
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
International Journal of Molecular Sciences, Vol 20, Iss 7, p 1688 (2019)
International Journal of Molecular Sciences
Volume 20
Issue 7
Popis: Rab GTPases define the identity and destiny of vesicles. Some of these small GTPases present isoforms that are expressed differentially along developmental stages or in a tissue-specific manner, hence comparative analysis is difficult to achieve. Here, we describe the intracellular distribution and function in lipid transport of the poorly characterized Rab39 isoforms using typical cell biology experimental tools and new ones developed in our laboratory. We show that, despite their amino acid sequence similarity, Rab39a and Rab39b display non-overlapping intracellular distribution. Rab39a localizes in the late endocytic pathway, mainly at multivesicular bodies. In contrast, Rab39b distributes in the secretory network, at the endoplasmic reticulum/cis-Golgi interface. Therefore, Rab39a controls trafficking of lipids (sphingomyelin and phospholipids) segregated at multivesicular bodies, whereas Rab39b transports sphingolipids biosynthesized at the endoplasmic reticulum-Golgi factory. Interestingly, lyso bis-phosphatidic acid is exclusively transported by Rab39a, indicating that both isoforms do not exert identical functions in lipid transport. Conveniently, the requirement of eukaryotic lipids by the intracellular pathogen Chlamydia trachomatis rendered useful for dissecting and distinguishing Rab39a-and Rab39b-controlled trafficking pathways. Our findings provide comparative insights about the different subcellular distribution and function in lipid transport of the two Rab39 isoforms. Fil: Gambarte Tudela, Julian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Buonfigli, Julio Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Luján, Lucas Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Alonso Bivou, Mariano Ángel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Cebrián, José Ignacio. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mendoza. Instituto de Histologia y Embriologia de Mendoza Dr. Mario H. Burgos. Grupo Vinculado de Investigacion y Desarrollo Biotecnologico Aplicado Al Diagnostico Al Ihem | Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Histologia y Embriologia de Mendoza Dr. Mario H. Burgos. Grupo Vinculado de Investigacion y Desarrollo Biotecnologico Aplicado Al Diagnostico Al Ihem.; Argentina Fil: Capmany, Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Damiani, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Databáze: OpenAIRE
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