The effect of omega-3 polyunsaturated fatty acids on the inflammatory response of the amnion
| Autor: | Simon C. Riley, Lorraine Frew, J He, Rosemary Leask, Jane E. Norman, N.U. Sugiarto, Sarah J. Stock, Shalini P. Rajagopal |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Clinical Biochemistry Anti-Inflammatory Agents Drug Evaluation Preclinical Inflammation Tissue Culture Techniques Troglitazone Pregnancy Internal medicine Fatty Acids Omega-3 medicine Humans Anilides Interleukin 8 Amnion Chromans Interleukin 6 health care economics and organizations chemistry.chemical_classification biology Parturition food and beverages Cell Biology Eicosapentaenoic acid PPAR gamma Endocrinology medicine.anatomical_structure Cytokine Biochemistry chemistry Matrix Metalloproteinase 9 Docosahexaenoic acid biology.protein Cytokines Matrix Metalloproteinase 2 lipids (amino acids peptides and proteins) Female Thiazolidinediones medicine.symptom Polyunsaturated fatty acid |
| Zdroj: | Prostaglandins, leukotrienes, and essential fatty acids. 89(4) |
| ISSN: | 1532-2823 |
| Popis: | To investigate the effect of omega-3 PUFAs, eicosapentanoic acid (EPA) and docosohexanoic acid (DHA) on inflammatory cytokine production in the amnion.Amnion explants were obtained at elective caesarean sections and cultured in vitro with EPA and DHA. IL-8 and IL-6 secretion was determined by ELISA, the role of PPARγ was investigated using specific agonists and antagonists and activity of MMP assessed by gelatin zymography.A combination of EPA and DHA significantly reduced the concentration of IL-8 and IL-6 released into the supernatant compared to untreated controls (p0.001). Stimulation of PPARγ with troglitazone reduced IL-8 production, and the PPARγ antagonist GW9662 partially reversed this effect. The activity of MMP-9 was also significantly reduced by treatment with EPA and DHA in combination compared to untreated control (p0.05).The omega-3 PUFAs EPA and DHA decrease the inflammatory response of the amnion, and this may be partially mediated through PPARγ. |
| Databáze: | OpenAIRE |
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