Preliminary characterization of two atypical soluble guanylyl cyclases in the central and peripheral nervous system ofDrosophila melanogaster

Autor: David B. Morton, Judith A. Stewart, Kristofor K. Langlais
Rok vydání: 2004
Předmět:
Physiology
Protein subunit
Molecular Sequence Data
Receptors
Cytoplasmic and Nuclear
Heterologous
Aquatic Science
Biology
Nitric Oxide
Nervous System
Gene Expression Regulation
Enzymologic
Soluble Guanylyl Cyclase
medicine
Animals
Amino Acid Sequence
Molecular Biology
In Situ Hybridization
Phylogeny
Ecology
Evolution
Behavior and Systematics
DNA Primers
chemistry.chemical_classification
Reverse Transcriptase Polymerase Chain Reaction
Activator (genetics)
Sequence Analysis
DNA
Blotting
Northern
biology.organism_classification
Immunohistochemistry
Cell biology
Amino acid
Alternative Splicing
Drosophila melanogaster
medicine.anatomical_structure
chemistry
Biochemistry
Guanylate Cyclase
Manduca sexta
Larva
Insect Science
Peripheral nervous system
Animal Science and Zoology
Soluble guanylyl cyclase
Sequence Alignment
Zdroj: Journal of Experimental Biology. 207:2323-2338
ISSN: 1477-9145
0022-0949
DOI: 10.1242/jeb.01025
Popis: SUMMARYConventional soluble guanylyl cyclases form α/β heterodimers that are activated by nitric oxide (NO). Recently, atypical members of the soluble guanylyl cyclase family have been described that include the ratβ2 subunit and MsGC-β3 from Manduca sexta. Predictions from the Drosophila melanogaster genome identify three atypical guanylyl cyclase subunits: Gyc-88E (formerly CG4154), Gyc-89Da (formerly CG14885) and Gyc-89Db (formerly CG14886). Preliminary data showed that transient expression of Gyc-88E in heterologous cells generated enzyme activity in the absence of additional subunits that was slightly stimulated by the NO donor sodium nitroprusside (SNP) but not the NO donor DEA-NONOate or the NO-independent activator YC-1. Gyc-89Db was inactive when expressed alone but when co-expressed with Gyc-88E enhanced the basal and SNP-stimulated activity of Gyc-88E, suggesting that they may form heterodimers in vivo. Here,we describe the localization of Gyc-88E and Gyc-89Db and show that they are expressed in the embryonic and larval central nervous systems and are colocalized in several peripheral neurons that innervate trachea, basiconical sensilla and the sensory cones in the posterior segments of the embryo. We also show that there are two splice variants of Gyc-88E that differ by seven amino acids, although no differences in biochemical properties could be determined. We have also extended our analysis of the NO activation of Gyc-88E and Gyc-89Db, showing that several structurally unrelated NO donors activate Gyc-88E when expressed alone or when co-expressed with Gyc-89Db.
Databáze: OpenAIRE
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