Expression of leptin receptor by glial cells of the nucleus tractus solitarius: possible involvement in energy homeostasis
| Autor: | André Jean, Emilie Pecchi, Jean-Denis Troadec, Michel Dallaporta, Juliette Pio, Kathleen C. Horner |
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| Přispěvatelé: | Centre de recherche en neurobiologie - neurophysiologie de Marseille (CRN2M), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Leptin
Male Stilbamidines Endocrinology Diabetes and Metabolism Gastric motility MESH: Protein Isoforms Energy homeostasis 0302 clinical medicine Endocrinology MESH: Stilbamidines Homeostasis Protein Isoforms MESH: Animals 2. Zero hunger 0303 health sciences MESH: Solitary Nucleus Area postrema digestive oral and skin physiology MESH: Energy Metabolism Brain MESH: Nutritional Status medicine.anatomical_structure Hypothalamus MESH: Homeostasis Neuroglia Receptors Leptin MESH: Neuroglia medicine.medical_specialty MESH: Rats Nutritional Status MESH: Receptors Leptin Biology 03 medical and health sciences Cellular and Molecular Neuroscience MESH: Brain Internal medicine medicine Solitary Nucleus Animals Rats Wistar [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] 030304 developmental biology Leptin receptor Endocrine and Autonomic Systems Solitary nucleus MESH: Rats Wistar MESH: Leptin MESH: Male Rats nervous system Energy Metabolism 030217 neurology & neurosurgery |
| Zdroj: | Journal of Neuroendocrinology Journal of Neuroendocrinology, Wiley, 2009, 21 (1), pp.57-67. ⟨10.1111/j.1365-2826.2008.01799.x⟩ Journal of Neuroendocrinology, 2009, 21 (1), pp.57-67. ⟨10.1111/j.1365-2826.2008.01799.x⟩ |
| ISSN: | 0953-8194 1365-2826 |
| Popis: | International audience; Leptin, an adipocyte-derived hormone, regulates food intake and body weight by acting principally on the hypothalamus, which displays the highest expression of leptin receptor (Ob-R). Nevertheless, other regions of the brain express Ob-R and constitute leptin's target sites. The dorsal vagal complex (DVC), an integrative centre of autonomic functions located in the caudal brainstem, is one of these structures. Leptin, by acting through the DVC, affects autonomic and neuroendocrine functions, such as control of food intake and gastric motility. In the present study, we observed Ob-R labelling within the DVC in cells that correspond to neuronal cell bodies. We showed for the first time Ob-R expression in a subpopulation of glial fibrillary acid protein positive cells located at the border between the area postrema and the nucleus tractus solitarius (NTS). These glial cells exhibit an atypical morphology consisting of unbranched processes that radiate rostro-caudally from the fourth ventricle wall. In vitro, the glial cells exhibited both long and short Ob-R expression with a preferential expression of the Ob-Ra and-f isoforms. Interestingly, using i.v and i.c.v. injection of the fluorescent tracer hydroxystilbamidine, we provided evidence that these cells may constitute a diffusion barrier which might regulate entry of molecules into the NTS. Finally, modulation of energy status, by acute or chronic reduction of food intake, modulated especially the short Ob-R isoforms in the DVC. In the light of these results, we hypothesise that Ob-R positive glial cells of the DVC participate in the transport of leptin into the brainstem and thus contribute to regulation of energy homeostasis. |
| Databáze: | OpenAIRE |
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