Intracellular and extracellular S100A9 trigger epithelial-mesenchymal transition and promote the invasive phenotype of pituitary adenoma through activation of AKT1
| Autor: | Guanjian Zhao, Ning Huang, Jiahe Tan, Jin Chen, Jiqin Zhang, Qiang Yang, Ying Tan, Yuan Cheng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Aging Epithelial-Mesenchymal Transition AKT1 Vimentin pituitary adenoma S100A9 Cell Movement Cell Line Tumor Extracellular Calgranulin B Humans Pituitary Neoplasms Epithelial–mesenchymal transition Cell adhesion Cell Proliferation biology Chemistry Cell Biology Middle Aged invasion Gene Expression Regulation Neoplastic Tumor progression biology.protein Cancer research Female Proto-Oncogene Proteins c-akt Intracellular Research Paper |
| Zdroj: | Aging (Albany NY) |
| ISSN: | 1945-4589 |
| Popis: | Pituitary adenoma (PA) is mostly benign intracranial tumor, but it also displays invasive growth characteristics and provokes challenging clinical conditions. S100A9 protein enhances tumor progression. In this study, we firstly demonstrated that both intracellular and extracellular S100A9 promoted the expression of Vimentin and Intercellular cell adhesion molecule-1 (ICAM-1), coupled with reduced E-cadherin in PA. As a result, PA acquired the phenotype of Epithelial-Mesenchymal Transition (EMT), leading to proliferation, cell cycle progression, migration and invasion. In addition, we indicated S100A9-induced EMT was mediated by activation of AKT1. Furthermore, immunohistochemistry showed that S100A9 expression was higher in invasive PA than that in non-invasive PA. These data extended our understanding for the effects of S100A9 on PA invasion and contributed to further development of a promising therapeutic target for invasive PA. |
| Databáze: | OpenAIRE |
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