Effects of a high-sodium/low-potassium diet on renal calcium, magnesium, and phosphate handling
| Autor: | Alexander H. J. Danser, Jenny van der Wijst, Caro Bos, Omar A. Z. Tutakhel, Joost G. J. Hoenderop, René J. M. Bindels, Ewout J. Hoorn |
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| Přispěvatelé: | Internal Medicine |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Time Factors Physiology Sodium 030232 urology & nephrology chemistry.chemical_element Calcium Phosphates 03 medical and health sciences 0302 clinical medicine TheoryofComputation_ANALYSISOFALGORITHMSANDPROBLEMCOMPLEXITY Internal medicine medicine Animals Homeostasis Magnesium Distal convoluted tubule Sodium Chloride Dietary Kidney Tubules Distal Kidney Reabsorption Membrane Transport Proteins Potassium Dietary Renal Reabsorption Animal Feed Mice Inbred C57BL Renal disorders Radboud Institute for Molecular Life Sciences [Radboudumc 11] 030104 developmental biology medicine.anatomical_structure Endocrinology Gene Expression Regulation chemistry Cotransporter |
| Zdroj: | American Journal of Physiology : Renal Physiology, 315, F110-F122 American Journal of Physiology-Renal Physiology, 315(1), F110-F122. American Physiological Society American Journal of Physiology : Renal Physiology, 315, 1, pp. F110-F122 |
| ISSN: | 1931-857X |
| Popis: | The distal convoluted tubule (DCT) of the kidney plays an important role in blood pressure regulation by modulating Na+reabsorption via the Na+-Cl−cotransporter (NCC). A diet containing high salt (NaCl) and low K+activates NCC, thereby causing Na+retention and a rise in blood pressure. Since high blood pressure, hypertension, is associated with changes in serum calcium (Ca2+) and magnesium (Mg2+) levels, we hypothesized that dietary Na+and K+intake affects Ca2+and Mg2+transport in the DCT. Therefore, the present study aimed to investigate the effect of a high-Na+/low-K+diet on renal Ca2+and Mg2+handling. Mice were divided in four groups and fed a normal-Na+/normal-K+, normal-Na+/low-K+, high-Na+/normal-K+, or high-Na+/low-K+diet for 4 days. Serum and urine were collected for electrolyte and hormone analysis. Gene and protein expression of electrolyte transporters were assessed in kidney and intestine by qPCR and immunoblotting. Whereas Mg2+homeostasis was not affected, the mice had elevated urinary Ca2+and phosphate (Pi) excretion upon high Na+intake, as well as significantly lower serum Ca2+levels in the high-Na+/low-K+group. Alterations in the gene and protein expression of players involved in Ca2+and Pitransport indicate that reabsorption in the proximal tubular and TAL is affected, while inducing a compensatory response in the DCT. These effects may contribute to the negative health impact of a high-salt diet, including kidney stone formation, chronic kidney disease, and loss of bone mineral density. |
| Databáze: | OpenAIRE |
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