Effect of cyclosporine on oxidative phosphorylation and adenylate energy charge of regenerating rat liver
| Autor: | K. Asonuma, Tohru Hashida, Ryohei Hori, Shinji Uemoto, Ken-ichi Inui, S. Matsuoka, Y. Kitakado, Nobuhiro Ozaki, Ryuji Okamura, K. Tanaka, Kazue Ozawa |
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| Rok vydání: | 1989 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Adenylate kinase Cyclosporins Mitochondria Liver Oxidative phosphorylation Biology Mitochondrion Oxidative Phosphorylation Adenosine Triphosphate Internal medicine Cyclosporin a medicine Animals Hepatectomy Energy charge Adenine Nucleotides Rats Inbred Strains General Medicine Liver regeneration Adenosine Monophosphate Liver Regeneration Rats Kinetics Endocrinology Phosphorylation |
| Zdroj: | Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie. 189(5) |
| ISSN: | 0300-9130 |
| Popis: | The effect of cyclosporin A (CyA) on regenerating liver was investigated in subtotal hepatectomized rats treated with CyA in terms of mitochondrial phosphorylative activity, hepatic energy charge, and serum bilirubin levels. In the CyA-treated hepatectomized group, the energy charge decreased from normal control value of 0.857 to 0.782 at 6 h after hepatectomy. The decreased energy charge, however, gradually increased and returned to 0.842 at 48 h after hepatectomy with no significant changes being observed between CyA-treated and untreated hepatectomized groups. Phosphorylation rate in the CyA-untreated group increased to 142% of the normal control at 24 h and then decreased to 114% at 48 h after hepatectomy. By contrast, phosphorylation rate in the CyA-treated group increased to 144% of the normal control at 24 h, but remained at the high value of 132% (P less than 0.01; compared to the CyA-untreated group) even at 48 h after hepatectomy. Serum total bilirubin levels in the CyA-treated group were significantly higher than those in the CyA-untreated group during all experimental periods. We conclude that CyA does not exert a direct detrimental effect on mitochondrial function and that, despite the marked hyperbilirubinemia induced by CyA, the mitochondrial phosphorylative activity increases adaptively to provide sufficient energy for enhanced ATP-utilizing reactions in an early process of liver regeneration. |
| Databáze: | OpenAIRE |
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