Procyanidins fromVitis viniferaSeeds Inhibit the Respiratory Burst of Activated Human Neutrophils and Lysosomal Enzyme Release
| Autor: | Marina Carini, Rita Stefani, Giancarlo Aldini, Marica Ozioli, Roberto Maffei Facino |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Neutrophils
Pharmaceutical Science chemistry.chemical_element Calcium Catechin Neutrophil Activation Analytical Chemistry chemistry.chemical_compound Superoxides Drug Discovery Biflavonoids Humans Proanthocyanidins Vitis Glucuronidase Peroxidase Respiratory Burst Pharmacology Pancreatic Elastase biology Plant Extracts Superoxide Organic Chemistry Elastase Degranulation Biological activity Respiratory burst Complementary and alternative medicine Biochemistry chemistry Myeloperoxidase Seeds biology.protein Molecular Medicine Liberation Quercetin Lysosomes Phytotherapy |
| Zdroj: | Scopus-Elsevier Europe PubMed Central |
| ISSN: | 1439-0221 0032-0943 |
| DOI: | 10.1055/s-2001-18353 |
| Popis: | The inhibitory properties of procyanidins (a standardized oligomeric catechin fraction) from Vitis vinifera L. seeds on the respiratory burst and on the release of granule components myeloperoxidase, beta-glucuronidase and elastase were studied in activated human neutrophils. Procyanidins strongly inhibit superoxide generation with an IC(50) of 7.2 microM, through a direct scavenging of superoxide and prevent the release from calcium ionophore activated neutrophils of beta-glucuronidase (IC(50) = 13.9 microM), myeloperoxidase (IC(50) = 7.2 microM) and elastase (IC(50) = 5.4 microM). In addition they dose-dependently inhibit the activity of myeloperoxidase released from calcium ionophore-stimulated cells with an IC(50) value of 2 microM. The monomeric constitutive unit (+)-catechin was far less active than procyanidins in all the models tested. These results evidence that procyanidins efficiently restrain the inflammatory response of activated neutrophils in vitro and whenever absorbed in vivo can prevent their oxidative discharge at the site(s) of their adhesion. |
| Databáze: | OpenAIRE |
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