Comparative proteomics reveals essential mechanisms for osmotolerance in Gluconacetobacter diazotrophicus
| Autor: | Vivian Ribeiro Pimentel, Luciano de Souza Vespoli, Vanildo Silveira, Lucas Zanchetta Passamani, Fabiano Silva Soares, Leandro Fernandes Andrade, Gonçalo Apolinário de Souza Filho, Mariana Ramos Leandro, Julia Rosa Moreira |
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| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Proteome Osmotic shock Plant Development Mutagenesis (molecular biology technique) Biology Microbiology Polyethylene Glycols 03 medical and health sciences Osmotic Pressure Molecular Biology Gene 030304 developmental biology chemistry.chemical_classification 0303 health sciences 030306 microbiology Gene Expression Profiling Cell Membrane Fatty Acids Fatty acid General Medicine Plants biology.organism_classification Gluconacetobacter chemistry Biochemistry Osmoprotectant Cell envelope Transcriptome Bacteria Acetyl-CoA Carboxylase |
| Zdroj: | Research in Microbiology. 172:103785 |
| ISSN: | 0923-2508 |
| DOI: | 10.1016/j.resmic.2020.09.005 |
| Popis: | Plant growth-promoting bacteria are a promising alternative to improve agricultural sustainability. Gluconacetobacter diazotrophicus is an osmotolerant bacterium able to colonize several plant species, including sugarcane, coffee, and rice. Despite its biotechnological potential, the mechanisms controlling such osmotolerance remain unclear. The present study investigated the key mechanisms of resistance to osmotic stress in G. diazotrophicus. The molecular pathways regulated by the stress were investigated by comparative proteomics, and proteins essential for resistance were identified by knock-out mutagenesis. Proteomics analysis led to identify regulatory pathways for osmotic adjustment, de novo saturated fatty acids biosynthesis, and uptake of nutrients. The mutagenesis analysis showed that the lack of AccC protein, an essential component of de novo fatty acid biosynthesis, severely affected G. diazotrophicus resistance to osmotic stress. Additionally, knock-out mutants for nutrients uptake (Δtbdr and ΔoprB) and compatible solutes synthesis (ΔmtlK and ΔotsA) became more sensitive to osmotic stress. Together, our results identified specific genes and mechanisms regulated by osmotic stress in an osmotolerant bacterium, shedding light on the essential role of cell envelope and extracytoplasmic proteins for osmotolerance. |
| Databáze: | OpenAIRE |
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