Expression of Regeneration and Tolerance Factor Correlates Directly with Human Immunodeficiency Virus Infection and Inversely with Hepatitis C Virus Infection

Autor: Alice Gilman-Sachs, Jonathan S. Boomer, Kenneth D. Beaman, Maxwell P. Westerman, Brian K. DuChateau, C. C. Sung, M. R. Lepe, Tara S. Givens, Axel Feller, Antonio Chedid
Rok vydání: 2000
Předmět:
CD4-Positive T-Lymphocytes
Male
Microbiology (medical)
CD3 Complex
T-Lymphocytes
Hepatitis C virus
CD3
Clinical Biochemistry
Immunology
HIV Infections
CD8-Positive T-Lymphocytes
Pregnancy Proteins
CD38
Lymphocyte Activation
medicine.disease_cause
Mice
NAD+ Nucleosidase
Immune system
Downregulation and upregulation
Antigens
CD
TheoryofComputation_ANALYSISOFALGORITHMSANDPROBLEMCOMPLEXITY
Suppressor Factors
Immunologic
medicine
Animals
Humans
Immunology and Allergy
ADP-ribosyl Cyclase
Mice
Inbred BALB C
Membrane Glycoproteins
biology
virus diseases
HLA-DR Antigens
Hepatitis C
NAD+ nucleosidase
medicine.disease
ADP-ribosyl Cyclase 1
Antigens
Differentiation
Virology
digestive system diseases
Membrane glycoproteins
ComputingMethodologies_PATTERNRECOGNITION
Immune-Mediated Responses and Disorders
biology.protein
Female
Zdroj: Clinical Diagnostic Laboratory Immunology. 7:200-205
ISSN: 1098-6588
1071-412X
Popis: Hepatitis C virus (HCV) and human immunodeficiency virus (HIV) cause two of the most prevalent debilitating viral infections. HIV appears to induce a skewing toward a Th2 response, while in HCV infection a Th1 response appears to dominate. Regeneration and tolerance factor (RTF) may participate in driving or sustaining a Th2 cytokine response. The expression of RTF on CD3+T cells of HIV-seropositive (HIV+) individuals is increased. The purpose of this study was to compare the expression of RTF during HIV infections with that during HCV infections. Three-color flow-cytometric analysis of peripheral blood collected from HIV+HCV-seropositive (HCV+), HIV- and HCV-seropositive (HIV+HCV+), and HIV- and HCV-seronegative (HIV−HCV−) individuals was performed. Levels of RTF expression on T-lymphocyte subsets from these groups were compared, as were levels of RTF expression on activated T cells expressing CD38 and HLA-DR, to determine the relationship of RTF expression to these infections. We demonstrated that the expression of RTF on surfaces of T cells from HIV+individuals is upregulated and that its expression on T cells from HCV+individuals is downregulated. A twofold increase in the mean channel fluorescence of RTF on CD3+T cells was seen in both HIV+and HIV+HCV+individuals compared to HIV−HCV−individuals. HCV+individuals had lower levels of RTF expression than HIV−HCV−individuals (P< 0.005 for CD4+;P< 0.0005 for CD8+). In terms of percentages of T cells expressing RTF, the groups were ranked as follows: HIV+> HIV+HCV+> HIV−HCV−> HCV+. The results indicate that RTF expression correlates with HIV-associated immune activation and may be associated with Th2-type responses.
Databáze: OpenAIRE
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